ArticleAntimicrobial agents and chemotherapy2003
Intracellular expression of Peptide fusions for demonstration of protein essentiality in bacteria.
Article in Antimicrobial agents and chemotherapy, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 31 citations in OpenAlex.
- Phage Display-Derived Peptides and Antibodies for Bacterial Infectious Diseases Therapy and Diagnosis.Molecules (Basel, Switzerland) · 2023Review
- Targeting LPS biosynthesis and transport in gram-negative bacteria in the era of multi-drug resistance.Biochimica et biophysica acta. Molecular cell research · 2023Review
- Structure guided design of an antibacterial peptide that targets UDP-N-acetylglucosamine acyltransferase.Scientific reports · 2019Article
- New virulence factor CSK29544_02616 as LpxA binding partner in Cronobacter sakazakii.Scientific reports · 2018Article
- Structure, inhibition, and regulation of essential lipid A enzymes.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2017Review
- Lipid A as a Drug Target and Therapeutic Molecule.Biomolecules & therapeutics · 2015Review
- Structural basis for the recognition of peptide RJPXD33 by acyltransferases in lipid A biosynthesis.The Journal of biological chemistry · 2014Article
- TetR-based gene regulation systems for Francisella tularensis.Applied and environmental microbiology · 2012Article
- Dual targeting antibacterial peptide inhibitor of early lipid A biosynthesis.ACS chemical biology · 2012Article
- Engineered phage-based therapeutic materials inhibit Chlamydia trachomatis intracellular infection.Biomaterials · 2012Article
- Structural basis for the sugar nucleotide and acyl-chain selectivity of Leptospira interrogans LpxA.Biochemistry · 2009Article
- Concurrent growth rate and transcript analyses reveal essential gene stringency in Escherichia coli.PloS one · 2009Article
- Structural basis for the acyl chain selectivity and mechanism of UDP-N-acetylglucosamine acyltransferase.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- The plasmid RK2 replication initiator protein (TrfA) binds to the sliding clamp beta subunit of DNA polymerase III: implication for the toxicity of a peptide derived from the amino-terminal portion of 33-kilodalton TrfA.Journal of bacteriology · 2006Article
- Structure of UDP-N-acetylglucosamine acyltransferase with a bound antibacterial pentadecapeptide.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We describe a "protein knockout" technique that can be used to identify essential proteins in bacteria. This technique uses phage display to select peptides that bind specifically to purified target proteins. The peptides are expressed intracellularly and cause inhibition of growth when the protein is essential. In this study, peptides that each specifically bind to one of seven essential proteins were identified by phage display and then expressed as fusions to glutathione S-transferase in Escherichia coli. Expression of peptide fusions directed against E. coli DnaN, LpxA, RpoD, ProRS, SecA, GyrA, and Era each dramatically inhibited cell growth. Under the same conditions, a fusion with a randomized peptide sequence did not inhibit cell growth. In growth-inhibited cells, inhibition could be relieved by concurrent overexpression of the relevant target protein but not by coexpression of an irrelevant protein, indicating that growth inhibition was due to a specific interaction of the expressed peptide with its target. The protein knockout technique can be used to assess the essentiality of genes of unknown function emerging from the sequencing of microbial genomes. This technique can also be used to validate proteins as drug targets, and their corresponding peptides as screening tools, for discovery of new antimicrobial agents.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.