ArticleInfection and immunity2003
Characterization of Pseudomonas aeruginosa exoenzyme S as a bifunctional enzyme in J774A.1 macrophages.
Article in Infection and immunity, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 62 citations in OpenAlex.
- Pycnogenol reduces the expression of P. aeruginosa T3SS and inflammatory response in NCI-H292 cells.Journal of microbiology (Seoul, Korea) · 2025Article
- Unravelling the Roles of Bacterial Nanomachines Bistability in Pathogens' Life Cycle.Microorganisms · 2024Review
- Emergence of hypervirulenthLife · 2023Article
- α-Helices in the Type III Secretion Effectors: A Prevalent Feature with Versatile Roles.International journal of molecular sciences · 2021Review
- Review
- Pseudomonas aeruginosa ExsA Regulates a Metalloprotease, ImpA, That Inhibits Phagocytosis of Macrophages.Infection and immunity · 2019Article
- MAV_4644 Interaction with the Host Cathepsin Z ProtectsMicroorganisms · 2019Article
- Design and characterization of a polyamine derivative inhibiting the expression of type III secretion system in Pseudomonas aeruginosa.Scientific reports · 2016Article
- Pseudomonas aeruginosa: breaking down barriers.Current genetics · 2016Review
- Urinary tract infections: epidemiology, mechanisms of infection and treatment options.Nature reviews. Microbiology · 2015Review
- Article
- The impairment of methylmenaquinol:fumarate reductase affects hydrogen peroxide susceptibility and accumulation in Campylobacter jejuni.MicrobiologyOpen · 2014Article
- In vitro assays to monitor the activity of Pseudomonas aeruginosa Type III secreted proteins.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Regulation of Rab5 function during phagocytosis of live Pseudomonas aeruginosa in macrophages.Infection and immunity · 2013Article
- Examining the role of actin-plasma membrane association in Pseudomonas aeruginosa infection and type III secretion translocation in migratory T24 epithelial cells.Infection and immunity · 2012Article
- Innate immune responses to Pseudomonas aeruginosa infection.Microbes and infection · 2011Review
- Pseudomonas aeruginosa population structure revisited.PloS one · 2009Article
- The type III secretion system of Pseudomonas aeruginosa: infection by injection.Nature reviews. Microbiology · 2009Review
- Modulation of host cell endocytosis by the type III cytotoxin, Pseudomonas ExoS.Traffic (Copenhagen, Denmark) · 2008Article
- Persistent infection with Pseudomonas aeruginosa in ventilator-associated pneumonia.American journal of respiratory and critical care medicine · 2008Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Pseudomonas aeruginosa exoenzyme S (ExoS) is a type III secretion (TTS) effector, which includes both a GTPase-activating protein (GAP) activity toward the Rho family of low-molecular-weight G (LMWG) proteins and an ADP-ribosyltransferase (ADPRT) activity that targets LMWG proteins in the Ras, Rab, and Rho families. The coordinate function of both activities of ExoS in J774A.1 macrophages was assessed by using P. aeruginosa strains expressing and translocating wild-type ExoS or ExoS defective in GAP and/or ADPRT activity. Distinct and coordinated functions were identified for both domains. The GAP activity was required for the antiphagocytic effect of ExoS and was linked to interference of lamellopodium and membrane ruffle formation. Alternatively, the ADPRT activity of ExoS altered cellular adherence and morphology and was linked to effects on filopodium formation. The cellular mechanism of ExoS GAP activity included an inactivation of Rac1 function, as determined in p21-activated kinase 1-glutathione S-transferase (GST) pull-down assays. The ADPRT activity of ExoS targeted Ras and RalA but not Rab or Rho proteins, and Ral binding protein 1-GST pull-down assays identified an effect of ExoS ADPRT activity on RalA activation. The results from these studies confirm the bifunctional nature of ExoS activity within macrophages when translocated by TTS.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.