ArticleInfection and immunity2003
Early induction of humoral and cellular immune responses during experimental Mycobacterium avium subsp. paratuberculosis infection of calves.
Article in Infection and immunity, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed.
- A Longitudinal Study ofAnimals : an open access journal from MDPI · 2026Article
- Evaluation of three ELISA assays for detection of Mycobacterium avium subsp. paratuberculosis antibodies in serum of sika deer (Cervus nippon).Scientific reports · 2026Article
- Sheep challenged with sheep-derived type II Mycobacterium avium subsp. paratuberculosis: the first experimental model of paratuberculosis in China.BMC veterinary research · 2025Article
- Interferon-gamma producing CD4Veterinary research · 2024Article
- Review
- Article
- Evaluation of a droplet digital PCR assay for quantification ofFrontiers in veterinary science · 2022Article
- Advances in Understanding of the Immune Response to Mycobacterial Pathogens and Vaccines through Use of Cattle andVaccines · 2021Review
- Vitamin d and leishmaniasis: Neither seasonal nor risk factor in canine host but potential adjuvant treatment through cbd103 expression.PLoS neglected tropical diseases · 2021Article
- Influence of Heterologous and Homologous Vaccines, and Their Components, on the Host Immune Response and Protection Against Experimental Caprine Paratuberculosis.Frontiers in veterinary science · 2021Article
- Regional Dichotomy in Enteric Mucosal Immune Responses to a PersistentFrontiers in immunology · 2020Article
- A Bovine EntericFrontiers in immunology · 2020Article
- The humoral immune response is essential for successful vaccine protection against paratuberculosis in sheep.BMC veterinary research · 2019Article
- Prevalence of Mycobacterium avium subspecies paratuberculosis IS 900 DNA in biopsy tissues from patients with Crohn's disease: histopathological and molecular comparison with Johne's disease in Fars province of Iran.BMC infectious diseases · 2019Article
- Membrane and Cytoplasmic Proteins ofMicroorganisms · 2018Article
- Resilience to infection by Mycobacterium avium subspecies paratuberculosis following direct intestinal inoculation in calves.Veterinary research · 2018Article
- A Mycobacterium avium subsp. paratuberculosis relA deletion mutant and a 35 kDa major membrane protein elicit development of cytotoxic T lymphocytes with ability to kill intracellular bacteria.Veterinary research · 2018Article
- Recombinant 20.8-kDa protein of Mycobacterium avium subsp. paratuberculosis-based sero-diagnosis of paratuberculosis.3 Biotech · 2017Article
- Fecal shedding and tissue infections demonstrate transmission of Mycobacterium avium subsp. paratuberculosis in group-housed dairy calves.Veterinary research · 2017Article
- Early detection of Mycobacterium avium subsp. paratuberculosis infection in cattle with multiplex-bead based immunoassays.PloS one · 2017Article
12 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Johne's disease (paratuberculosis) of cattle is widespread and causes significant economic losses for producers due to decreased production and poor health of affected animals. The chronic nature of the disease and the lack of a reproducible model of infection hinder research efforts. In the present study, instillation of Mycobacterium avium subsp. paratuberculosis into the tonsillar crypts of neonatal calves resulted in peripheral colonization as detected by antemortem culture of feces and postmortem (320 days postchallenge) culture of intestinal tissues. Antigen-specific blastogenic, gamma interferon (IFN-gamma), and nitric oxide responses by blood mononuclear cells from infected calves exceeded prechallenge responses beginning 194 days postchallenge. Upon in vitro stimulation with paratuberculosis antigens, CD4(+) cells from infected calves proliferated, produced IFN-gamma, and increased expression of CD26 and CD45RO (indicative of an activated memory phenotype). Utilizing a lipoarabinomannan-based enzyme-linked immunosorbent assay, specific serum immunoglobulin was detected as early as 134 days postchallenge and generally increased after this time point. Two antigens of approximately 50 and approximately 60 kDa were particularly immunodominant early in infection, as shown by immunoblot with serum collected within 2 weeks postchallenge. Findings indicate that the intratonsillar inoculation route will prove useful as an experimental model for paratuberculosis infection. Additionally, this study confirms that mycobacteria-specific antibody is detectable early in the course of experimental Johne's disease, even preceding the development of specific cell-mediated responses.
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