ArticleInfection and immunity2003
Stimulation of T-helper cell gamma interferon and immunoglobulin G responses specific for Babesia bovis rhoptry-associated protein 1 (RAP-1) or a RAP-1 protein lacking the carboxy-terminal repeat region is insufficient to provide protective immunity against virulent B. bovis challenge.
Article in Infection and immunity, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Evaluation of the Use of Sub-Immunodominant Antigens ofVaccines · 2024Article
- A conserved motif in the immune-subdominant RAP-1 related antigen ofFrontiers in immunology · 2024Article
- Multi-epitope Based Peptide Vaccine Candidate AgainstBioinformatics and biology insights · 2024Article
- Current vaccines, experimental immunization trials, and new perspectives to control selected vector borne blood parasites of veterinary importance.Frontiers in veterinary science · 2024Review
- Developing Anti-International journal of molecular sciences · 2023Review
- Compilation of parasitic immunogenic proteins from 30 years of published research using machine learning and natural language processing.Scientific reports · 2022Article
- Applying Machine Learning to Predict the Exportome of Bovine and CaninePathogens (Basel, Switzerland) · 2021Article
- Predicting Protein Therapeutic Candidates for Bovine Babesiosis Using Secondary Structure Properties and Machine Learning.Frontiers in genetics · 2021Article
- Immune Response to Tick-Borne Hemoparasites: Host Adaptive Immune Response Mechanisms as Potential Targets for Therapies and Vaccines.International journal of molecular sciences · 2020Review
- An Overview of Current Knowledge onFrontiers in veterinary science · 2020Review
- Variable and Variant Protein Multigene Families inPathogens (Basel, Switzerland) · 2019Review
- Immune Stimulation of RAP domain binding protein (rTgRA15) from Toxoplasma gondii.Pathogens and global health · 2018Article
- A recombinant multi-antigen vaccine formulation containing Babesia bovis merozoite surface antigens MSA-2aParasites & vectors · 2016Article
- CD4 T cell antigens from Staphylococcus aureus Newman strain identified following immunization with heat-killed bacteria.Clinical and vaccine immunology : CVI · 2012Article
- Anaplasma marginale type IV secretion system proteins VirB2, VirB7, VirB11, and VirD4 are immunogenic components of a protective bacterial membrane vaccine.Infection and immunity · 2010Article
- Rapid deletion of antigen-specific CD4+ T cells following infection represents a strategy of immune evasion and persistence for Anaplasma marginale.Journal of immunology (Baltimore, Md. : 1950) · 2008Article
- Immunogenicity of Anaplasma marginale type IV secretion system proteins in a protective outer membrane vaccine.Infection and immunity · 2007Article
- Quantitation of Anaplasma marginale major surface protein (MSP)1a and MSP2 epitope-specific CD4+ T lymphocytes using bovine DRB3*1101 and DRB3*1201 tetramers.Immunogenetics · 2006Article
- Intrahaplotype and interhaplotype pairing of bovine leukocyte antigen DQA and DQB molecules generate functional DQ molecules important for priming CD4(+) T-lymphocyte responses.Immunogenetics · 2005Article
- Conservation of Babesia bovis small heat shock protein (Hsp20) among strains and definition of T helper cell epitopes recognized by cattle with diverse major histocompatibility complex class II haplotypes.Infection and immunity · 2004Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Rhoptry-associated protein 1 (RAP-1) is a targeted vaccine antigen for Babesia bovis and Babesia bigemina infections of cattle. The 60-kDa B. bovis RAP-1 is recognized by antibodies and T lymphocytes from cattle that recovered from infection and were immune to subsequent challenge. Immunization with native or recombinant protein was reported to reduce parasitemias in challenged animals. We recently reported that the NT domain of B. bovis RAP-1 contained immunodominant T-cell epitopes, whereas the repeat-rich CT domain was less immunostimulatory for T lymphocytes from cattle immune to B. bovis. The present study was therefore designed to test the hypothesis that the NT region of RAP-1, used as a vaccine with interleukin-12 and RIBI (catalog no. R-730; RIBI Immunochem Research, Inc., Hamilton, Mont. [now Corixa, Seattle, Wash.]) adjuvant to induce a type 1 response, would prime calves for antibody and T-helper cell responses comparable to or greater than those induced by full-length RAP-1 containing the C-terminal repeats. Furthermore, a type 1 immune response to RAP-1 was hypothesized to induce protection against challenge. Following four inoculations of either recombinant full-length RAP-1 or RAP-1 NT protein, RAP-1-specific immunoglobulin G (IgG) titers, T-lymphocyte proliferation, and gamma interferon production were similar. Similar numbers of NT region peptides were recognized. However, in spite of the presence of strong RAP-1-specific IgG and CD4(+)-T-lymphocyte responses that were recalled upon challenge, neither antigen stimulated a protective immune response. We conclude that successful priming of calves with recombinant RAP-1 and adjuvants that elicit strong Th1 cell and IgG responses is insufficient to protect calves against virulent B. bovis challenge.
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