ArticleInfection and immunity2003
Multiple elements controlling adherence of enterohemorrhagic Escherichia coli O157:H7 to HeLa cells.
Article in Infection and immunity, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
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Who cites it
75 citing papers in PubMed.
- Temporal and Spatial Profiling ofProteomes · 2025Article
- Deoxynivalenol modulated mucin expression and proinflammatory cytokine production, affecting susceptibility to enteroinvasive Escherichia coli infection in intestinal epithelial cells.Journal of food science · 2025Article
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- Synthetic and natural antimicrobials as a control against food borne pathogens: A review.Heliyon · 2023Review
- Cellular RNA Targets of Cold Shock Proteins CspC and CspE and Their Importance for Serum Resistance in Septicemic Escherichia coli.mSystems · 2022Article
- Human Monoclonal Antibodies to Escherichia coli Outer Membrane Protein A Porin Domain Cause Aggregation but Do Not AlterInfection and immunity · 2022Article
- Roles of OmpA in Type III Secretion System-Mediated Virulence of EnterohemorrhagicPathogens (Basel, Switzerland) · 2021Article
- Diversification of OmpA and OmpF of Yersinia ruckeri is independent of the underlying species phylogeny and evidence of virulence-related selection.Scientific reports · 2021Article
- Effects of Essential Oils fromAntibiotics (Basel, Switzerland) · 2021Article
- Putative β-Barrel Outer Membrane Proteins of the Bovine Digital Dermatitis-Associated Treponemes: Identification, Functional Characterization, and Immunogenicity.Infection and immunity · 2020Article
- A genome-wide scan for genes under balancing selection in the plant pathogen Ralstonia solanacearum.BMC evolutionary biology · 2019Article
- Amino Acid Substitutions and Differential Gene Expression of Outer Membrane Proteins in Adherent-InvasiveFrontiers in microbiology · 2019Article
- Article
- Full-Length OmpA: Structure, Function, and Membrane Interactions Predicted by Molecular Dynamics Simulations.Biophysical journal · 2016Article
- Survival of the Fittest: How Bacterial Pathogens Utilize Bile To Enhance Infection.Clinical microbiology reviews · 2016Review
- Article
- Translatomics combined with transcriptomics and proteomics reveals novel functional, recently evolved orphan genes in Escherichia coli O157:H7 (EHEC).BMC genomics · 2016Article
- Immunization with a 22-kDa outer membrane protein elicits protective immunity to multidrug-resistant Acinetobacter baumannii.Scientific reports · 2016Article
- A Highly Conserved Bacterial D-Serine Uptake System Links Host Metabolism and Virulence.PLoS pathogens · 2016Article
- Trade-Offs of Escherichia coli Adaptation to an Intracellular Lifestyle in Macrophages.PloS one · 2016Article
15 more citing papers are in PubMed but not listed here.
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2 authors.
Funding
Abstract
Adherence of enterohemorrhagic Escherichia coli (EHEC) to the intestinal epithelium is essential for initiation of infection. Intimin is the only factor demonstrated to play a role in intestinal colonization by EHEC O157:H7. Other attempts to identify additional adhesion factors in vitro have been unsuccessful, suggesting that expression of these factors is under tight regulation. We sought to identify genes involved in the control of adherence of EHEC O157:H7 to cultured epithelial cells. A total of 5,000 independent transposon insertion mutants were screened for their ability to adhere to HeLa cells, and 7 mutants were isolated with a markedly enhanced adherence. The mutants adhered at levels 113 to 170% that of the wild-type strain, and analysis of the protein profiles of these mutants revealed several proteins differentially expressed under in vitro culture conditions. We determined the sequence of the differentially expressed proteins and further investigated the function of OmpA, whose expression was increased in a mutant with an insertionally inactivated tcdA gene. An isogenic ompA mutant showed reduced adherence compared to the parent strain. Disruption of the ompA gene in the tdcA mutant strain abolished the hyperadherent phenotype, and anti-OmpA serum inhibited adhesion of wild-type and tdcA mutant strains to HeLa cells. Enhanced adhesion mediated by OmpA was also observed with Caco-2 cells, and anti-OmpA serum blocked adherence to HeLa cells of other EHEC O157:H7 strains. Our results indicate that multiple elements control adherence and OmpA acts as an adhesin in EHEC O157:H7.
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