ArticleReproductive biology and endocrinology : RB&E2003
Identification of human and mouse CatSper3 and CatSper4 genes: characterisation of a common interaction domain and evidence for expression in testis.
Article in Reproductive biology and endocrinology : RB&E, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers, 2 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
66 citing papers in PubMed, 2 syntheses or guidelines pooled it, 152 citations in OpenAlex.
- The Catsper channel and its roles in male fertility: a systematic review.Reproductive biology and endocrinology : RB&E · 2017Pooled it
- Identification of nine new susceptibility loci for testicular cancer, including variants near DAZL and PRDM14.Nature genetics · 2013Pooled it
- Ion Channels as Gatekeepers of Fertility: from Uterine Kir7.1 to Sperm CatSper.Physiology (Bethesda, Md.) · 2027Review
- A histidine-mediated supramolecular mechanism links temperature and pH activation of the sperm channel CatSper.PLoS biology · 2026Article
- CATSPERβ-δ Interaction Governs Hierarchical CatSper Holo-complex Assembly and is Essential for Male Fertility.bioRxiv : the preprint server for biology · 2026Article
- The systems that transport ions and their impact on sperm function and male fertility.Function (Oxford, England) · 2026Review
- Article
- Molecular Characterization of the Murine Catsper4 Promoter and its Regulation by CREMτ.Reproductive sciences (Thousand Oaks, Calif.) · 2026Article
- Molecular basis of the higher-order assembly of CatSper.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Presence and Location of CatSper 1-4, Opioid (μ, δ and κ) and CD44 Receptors in SPERMATOZOA from AOUDAD, IBERIAN IBEX and Mouflon.Veterinary medicine and science · 2025Article
- Calcium Channels and Modulators as Potential Therapeutic Targets for Contraceptives and Male Fertility: A Scoping Review.Open access journal of contraception · 2025Review
- CATSPER2 and SPEF2 are potential molecular markers for boar sperm quality: a population association study.Journal of assisted reproduction and genetics · 2024Article
- From Bench to Babies - Drug Development for Male Subfertility.Reproduction & fertility · 2024Article
- Sperm hyperactivation and the CatSper channel: current understanding and future contribution of domestic animals.Journal of animal science and technology · 2024Review
- CaAnimal cells and systems · 2024Review
- The Cation/Calcium Channel of Sperm (CatSper): A Common Role Played Despite Inter-Species Variation?International journal of molecular sciences · 2023Review
- CatSper Calcium Channels: 20 Years On.Physiology (Bethesda, Md.) · 2023Review
- The Role of Sperm Membrane Potential and Ion Channels in Regulating Sperm Function.International journal of molecular sciences · 2023Review
- High-throughput screening method for discovering CatSper inhibitors using membrane depolarization caused by external calcium chelation and fluorescent cell barcoding.Frontiers in cell and developmental biology · 2023Article
- Characterization of the promoter region of the murine Catsper2 gene.FEBS open bio · 2022Article
6 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCatSper1 and CatSper2 are two recently identified channel-like proteins, which show sperm specific expression patterns. Through targeted mutagenesis in the mouse, CatSper1 has been shown to be required for fertility, sperm motility and for cAMP induced Ca2+ current in sperm. Both channels resemble a single pore forming repeat from a four repeat voltage dependent Ca2+ /Na+ channel. However, neither CatSper1 or CatSper2 have been shown to function as cation channels when transfected into cells, singly or in conjunction. As the pore forming units of voltage gated cation channels form a tetramer it has been suggested that the known CatSper proteins require additional subunits and/or interaction partners to function.
resultsUsing in silico gene identification and prediction techniques, we have identified two further members of the CatSper family, CatSper3 and Catsper4. Each carries a single channel-forming domain with the predicted pore-loop containing the consensus sequence TxDxW. Each of the new CatSper genes has evidence for expression in the testis. Furthermore we identified coiled-coil protein-protein interaction domains in the C-terminal tails of each of the CatSper channels, implying that CatSper channels 1,2,3 and 4 may interact directly or indirectly to form a functional tetramer.
conclusionsThe topological and sequence relationship of CatSper1 and CatSper2 to the four repeat Ca2+ /Na+ channels suggested other members of this family may exist. We have identified a further two novel CatSper genes, conserved in both the human and mouse genomes. Furthermore, all four of the CatSper proteins are predicted to contain a common coiled-coil protein-protein interaction domain in their C-terminal tail. Coupled with expression data this leads to the hypothesis that the CatSper proteins form a functional hetero-tetrameric channel in sperm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.