ArticleArthritis research & therapy2003
Higher susceptibility to Fas ligand induced apoptosis and altered modulation of cell death by tumor necrosis factor-alpha in periarticular tenocytes from patients with knee joint osteoarthritis.
Article in Arthritis research & therapy, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 39 citations in OpenAlex.
- Pooled it
- Severe knee osteoarthritis is associated with histological and ultrastructural changes in the semitendinosus tendon.Journal of experimental orthopaedics · 2025Article
- ADAM8 silencing suppresses the migration and invasion of fibroblast-like synoviocytes via FSCN1/MAPK cascade in osteoarthritis.Arthritis research & therapy · 2024Article
- Challenges in tendon-bone healing: emphasizing inflammatory modulation mechanisms and treatment.Frontiers in endocrinology · 2024Review
- Infrapatellar fat pad adipose tissue-derived macrophages display a predominant CD11c+CD206+ phenotype and express genotypes attributable to key features of OA pathogenesis.Frontiers in immunology · 2024Article
- Species variations in tenocytes' response to inflammation require careful selection of animal models for tendon research.Scientific reports · 2021Article
- Infliximab prevents systemic bone loss and suppresses tendon inflammation in a collagen-induced arthritis rat model.Inflammopharmacology · 2021Article
- Tectorigenin Alleviates Inflammation, Apoptosis, and Ossification in Rat Tendon-Derived Stem CellsFrontiers in cell and developmental biology · 2020Article
- Lose-Dose Administration of Dexamethasone Is Beneficial in Preventing Secondary Tendon Damage in a Stress-Deprived Joint Injury Explant Model.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2020Article
- The roles of inflammatory mediators and immunocytes in tendinopathy.Journal of orthopaedic translation · 2018Review
- Analysis of DNA methylation in chondrocytes in rats with knee osteoarthritis.BMC musculoskeletal disorders · 2017Article
- Shikonin inhibits inflammation and chondrocyte apoptosis by regulation of the PI3K/Akt signaling pathway in a rat model of osteoarthritis.Experimental and therapeutic medicine · 2016Article
- Substance P reduces TNF-α-induced apoptosis in human tenocytes through NK-1 receptor stimulation.British journal of sports medicine · 2014Article
- The effect of tenocyte/hyaluronic acid therapy on the early recovery of healing Achilles tendon in rats.Journal of materials science. Materials in medicine · 2014Article
- Non-immortalized human tenocyte cultures as a vehicle for understanding cellular aspects to tendinopathy.Translational medicine @ UniSa · 2011Article
- Tissue specific characteristics of cells isolated from human and rat tendons and ligaments.Journal of orthopaedic surgery and research · 2008Article
- Altered expression of membrane-bound and soluble CD95/Fas contributes to the resistance of fibrotic lung fibroblasts to FasL induced apoptosis.Respiratory research · 2005Article
- Reactive oxygen species and tendinopathy: do they matter?British journal of sports medicine · 2004Review
- Relationship between urokinase-type plasminogen activator receptor and vascular endothelial growth factor expression and metastasis of gallbladder cancer.World journal of gastroenterology · 2004Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of the present study was to investigate the expression of Fas in periarticular tenocytes of patients with osteoarthritis (OA) and to study their susceptibility to Fas ligand-mediated apoptosis. Tendon samples were obtained from the quadriceps femoris muscle of patients with knee OA and used for histological evaluation, for immunohistochemical detection of Fas, and to establish tenocyte cultures. The expression of Fas mRNA was determined by quantitative PCR. Levels of soluble Fas and soluble tumour necrosis factor (TNF) receptor I were measured using ELISA. Apoptosis was induced with recombinant human Fas ligand and measured by a histone fragmentation assay and flow cytometry. The effects of TNF-alpha were studied by stimulation with TNF-alpha alone or 24 hours before the induction of apoptosis. Tendon samples from non-OA patients were used as controls. Histological evaluation revealed degenerative changes in the tendons of all OA patients but not in the controls. Fas was detected by immunohistochemistry in all specimens, but quantitative PCR revealed significantly higher levels of Fas mRNA in OA tenocytes. In contrast, lower levels of soluble Fas were found in OA tenocytes by ELISA. OA tenocytes were significantly more susceptible to Fas ligand induced apoptosis than were control cells. TNF-alpha reduced the Fas ligand induced apoptosis in OA tenocytes but had no effects on control tenocytes. These data suggest that knee OA is associated with higher susceptibility of periarticular tenocytes to Fas ligand induced apoptosis because of higher expression of Fas but lower levels of apoptosis-inhibiting soluble Fas. These changes may contribute to decreased cellularity in degenerative tendons and promote their rupturing. The antiapoptotic effects of TNF-alpha in OA tenocytes most likely reflect regenerative attempts and must be taken into account when anti-TNF strategies are considered for OA.
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