ArticleNucleic acids research2003
Simultaneous gene expression analysis of steady-state and actively translated mRNA populations from osteosarcoma MG-63 cells in response to IL-1alpha via an open expression analysis platform.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 18 citations in OpenAlex.
- Preconditioning the rat heart with sodium thiosulfate preserved the mitochondria in response to ischemia-reperfusion injury.Journal of bioenergetics and biomembranes · 2019Article
- Human snoRNA-93 is processed into a microRNA-like RNA that promotes breast cancer cell invasion.NPJ breast cancer · 2017Article
- MiR-20a-5p represses multi-drug resistance in osteosarcoma by targeting the KIF26B gene.Cancer cell international · 2016Article
- Identification of miR-215 mediated targets/pathways via translational immunoprecipitation expression analysis (TrIP-chip).Oncotarget · 2015Article
- Informatics for RNA Sequencing: A Web Resource for Analysis on the Cloud.PLoS computational biology · 2015Article
- Prognostic significance of miR-215 in colon cancer.Clinical colorectal cancer · 2011Article
- Translational control analysis by translationally active RNA capture/microarray analysis (TrIP-Chip).Nucleic acids research · 2010Article
- Molecular mechanism of chemoresistance by miR-215 in osteosarcoma and colon cancer cells.Molecular cancer · 2010Article
- Identification of actively translated mRNA transcripts in a rat model of early-stage colon carcinogenesis.Cancer prevention research (Philadelphia, Pa.) · 2009Article
- Isolation of polysome-bound mRNA from solid tissues amenable for RT-PCR and profiling experiments.RNA (New York, N.Y.) · 2007Article
- Prognostic Values of microRNAs in Colorectal Cancer.Biomarker insights · 2006Article
- Immunopurification of polyribosomal complexes of Arabidopsis for global analysis of gene expression.Plant physiology · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pro-inflammatory cytokines play a key role in various forms of metabolic bone diseases, including osteopenia and osteoporosis. Human MG-63 cells treated with IL-1alpha were used as a model system to identify potential marker genes that are differentially expressed. This study is designed to quantitate gene expression of actively translated mRNAs as compared to the steady-state mRNA population. Both steady-state mRNAs and actively translated mRNAs from control MG-63 cells and MG-63 cells treated with IL-1alpha were isolated and converted to cDNA. The gene expression analysis from these samples was then quantitated with an open expression analysis platform with no requirement for a priori knowledge of sequence information. As a result, many differentially regulated genes were discovered via IL-1alpha treatment. Some of the genes have been described previously as playing important roles in the regulation of inflammation and cell adhesion. These comparisons provided a panoramic overview of gene expression at both the total transcript and post-transcriptional levels. In addition, the quantitation of actively translated mRNAs associated with polysomes also provided a better estimation of protein expression levels. This methodology allows for the identification of genes acutely regulated during translation. Furthermore, the process may aid in the identification of new drug targets or biomarkers.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.