ArticleNucleic acids research2003
Import of mitochondrial transcription factor A (TFAM) into rat liver mitochondria stimulates transcription of mitochondrial DNA.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 103 citations in OpenAlex.
- Mitochondrial Haplotype Shapes the Trajectory of Ovarian Aging in Genetically Heterogeneous Rats.Aging cell · 2026Article
- Activating AMPK improves pathological phenotypes due to mtDNA depletion.The FEBS journal · 2025Article
- 35 Years of TFAM Research: Old Protein, New Puzzles.Biology · 2023Review
- The C-Terminal Tail of Mitochondrial Transcription Factor A Is Dispensable for Mitochondrial DNA Replication and Transcription In Situ.International journal of molecular sciences · 2023Article
- Mitochondria-RelatedGenes · 2023Article
- The mitochondrial Ahi1/GR participates the regulation on mtDNA copy numbers and brain ATP levels and modulates depressive behaviors in mice.Cell communication and signaling : CCS · 2023Article
- Mitochondrial regulation in human pluripotent stem cells during reprogramming and β cell differentiation.Frontiers in endocrinology · 2023Review
- High levels of TFAM repress mammalian mitochondrial DNA transcription in vivo.Life science alliance · 2021Article
- Mitochondrial dysfunction in sepsis is associated with diminished intramitochondrial TFAM despite its increased cellular expression.Scientific reports · 2020Observational
- TGFβ-dependent mitochondrial biogenesis is activated during definitive endoderm differentiation.In vitro cellular & developmental biology. Animal · 2020Article
- Ginkgo biloba mitigates silver nanoparticles-induced hepatotoxicity in Wistar rats via improvement of mitochondrial biogenesis and antioxidant status.Environmental science and pollution research international · 2019Article
- Acetylation and phosphorylation of human TFAM regulate TFAM-DNA interactions via contrasting mechanisms.Nucleic acids research · 2018Article
- The mitochondrial transcription factor TFAM in neurodegeneration: emerging evidence and mechanisms.FEBS letters · 2018Review
- Resveratrol counteracts bone loss via mitofilin-mediated osteogenic improvement of mesenchymal stem cells in senescence-accelerated mice.Theranostics · 2018Article
- Increased hepatic mitochondrial FA oxidation reduces plasma and liver TG levels and is associated with regulation of UCPs and APOC-III in rats.Journal of lipid research · 2017Article
- Postnatal High-Fat Diet Increases Liver Steatosis and Apoptosis Threatened by Prenatal Dexamethasone through the Oxidative Effect.International journal of molecular sciences · 2016Article
- Mitochondrial transcript maturation and its disorders.Journal of inherited metabolic disease · 2015Review
- Analysis of mitochondrial transcription factor A SNPs in alcoholic cirrhosis.Experimental and therapeutic medicine · 2014Article
- Hepatocyte-like cells differentiated from human induced pluripotent stem cells: relevance to cellular therapies.Stem cell research · 2012Article
- Human mitochondrial transcription factor A induces a U-turn structure in the light strand promoter.Nature structural & molecular biology · 2011Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mitochondrial transcription factor A (TFAM) has been shown to stimulate transcription from mitochondrial DNA promoters in vitro. In order to determine whether changes in TFAM levels also regulate RNA synthesis in situ, recombinant human precursor proteins were imported into the matrix of rat liver mitochondria. After uptake of wt-TFAM, incorporation of [alpha-32P]UTP into mitochondrial mRNAs as well as rRNAs was increased 2-fold (P < 0.05), whereas import of truncated TFAM lacking 25 amino acids at the C-terminus had no effect. Import of wt-TFAM into liver mitochondria from hypothyroid rats stimulated RNA synthesis up to 4-fold. We conclude that the rate of transcription is submaximal in freshly isolated rat liver mitochondria and that increasing intra-mitochondrial TFAM levels is sufficient for stimulation. The low transcription rate associated with the hypothyroid state observed in vivo as well as in organello seems to be a result of low TFAM levels, which can be recovered by treating animals with T3 in vivo or by importing TFAM in organello. Thus, this protein meets the criteria for being a key factor in regulating mitochondrial gene expression in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.