ArticleProceedings of the National Academy of Sciences of the United States of America2003
Enhanced hematopoietic differentiation of embryonic stem cells conditionally expressing Stat5.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 83 citations in OpenAlex.
- Mechanometabolism instructs hematopoietic stem cell specification.The Journal of experimental medicine · 2026Article
- Investigation of materials for the development of new molecular and cellular antiviral and antimalignancy vaccines and immunization methods: a pilot study.Biotechnologia · 2025Article
- Mitochondrial permeability transition dictates mitochondrial maturation upon switch in cellular identity of hematopoietic precursors.Communications biology · 2024Article
- Host T Cell Dedifferentiation Effects Drive HIV-1 Latency Stability.Journal of virology · 2022Article
- Review
- Hierarchical decomposition of dynamically evolving regulatory networks.BMC bioinformatics · 2015Article
- Activated STAT5 confers resistance to intestinal injury by increasing intestinal stem cell proliferation and regeneration.Stem cell reports · 2015Article
- De novo formed satellite DNA-based mammalian artificial chromosomes and their possible applications.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Review
- Retroviral infection of murine embryonic stem cell derived embryoid body cells for analysis of hematopoietic differentiation.Journal of visualized experiments : JoVE · 2014Article
- Modeling murine yolk sac hematopoiesis with embryonic stem cell culture systems.Frontiers in biology · 2014Article
- Article
- Article
- Short Stat5-interacting peptide derived from phospholipase C-β3 inhibits hematopoietic cell proliferation and myeloid differentiation.PloS one · 2011Article
- Challenges and strategies for generating therapeutic patient-specific hemangioblasts and hematopoietic stem cells from human pluripotent stem cells.The International journal of developmental biology · 2010Review
- Toward clinical therapies using hematopoietic cells derived from human pluripotent stem cells.Blood · 2009Review
- Tumor suppression by phospholipase C-beta3 via SHP-1-mediated dephosphorylation of Stat5.Cancer cell · 2009Article
- A conserved role for Hox paralog group 4 in regulation of hematopoietic progenitors.Stem cells and development · 2009Article
- IL-3 induces basophil expansion in vivo by directing granulocyte-monocyte progenitors to differentiate into basophil lineage-restricted progenitors in the bone marrow and by increasing the number of basophil/mast cell progenitors in the spleen.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Mesp1 coordinately regulates cardiovascular fate restriction and epithelial-mesenchymal transition in differentiating ESCs.Cell stem cell · 2008Article
- Caspase activity mediates the differentiation of embryonic stem cells.Cell stem cell · 2008Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The signal transducer Stat5 plays a key role in the regulation of hematopoietic differentiation and hematopoietic stem cell function. To evaluate the effects of Stat5 signaling in the earliest hematopoietic progenitors, we have generated an embryonic stem cell line in which Stat5 signaling can be induced with doxycycline. Ectopic Stat5 activation at the point of origin of the hematopoietic lineage (from day 4 to day 6 of embryoid body differentiation) significantly enhances the number of hematopoietic progenitors with colony-forming potential. It does so without significantly altering total numbers or apoptosis of hematopoietic cells, suggesting a cell-intrinsic effect of Stat5 on either the developmental potential or clonogenicity of this population. From day-6 embryoid bodies, under the influence of Stat5 signaling, a population of semiadherent cells can be expanded on OP9 stromal cells that is comprised of primitive hematopoietic blast cells with ongoing, mainly myeloid, differentiation. When these cells are injected into lethally irradiated mice, they engraft transiently in a doxycycline-dependent manner. These results demonstrate that the hematopoietic commitment of embryonic stem cells may be augmented by a Stat5-mediated signal, and highlight the utility of manipulating individual components of signaling pathways for engineering tissue-specific differentiation of stem cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.