ArticleGenetics2003
Molecular markers for rapidly identifying candidate genes in Chlamydomonas reinhardtii. Ery1 and ery2 encode chloroplast ribosomal proteins.
Article in Genetics, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- MAPINS, a Highly Efficient Detection Method That Identifies Insertional Mutations and Complex DNA Rearrangements.Plant physiology · 2018Article
- Macrolides rapidly inhibit red blood cell invasion by the human malaria parasite, Plasmodium falciparum.BMC biology · 2015Article
- Genetic and genomic approaches to identify genes involved in flagellar assembly in Chlamydomonas reinhardtii.Methods in cell biology · 2015Article
- The Chlamydomonas mutant pf27 reveals novel features of ciliary radial spoke assembly.Cytoskeleton (Hoboken, N.J.) · 2013Article
- Whole-Genome Sequencing to Identify Mutants and Polymorphisms in Chlamydomonas reinhardtii.G3 (Bethesda, Md.) · 2012Article
- Article
- Genetic and phenotypic analysis of flagellar assembly mutants in Chlamydomonas reinhardtii.Methods in cell biology · 2009Article
- Treasure hunting in the Chlamydomonas genome.Genetics · 2008Article
- Intrinsic and selected resistance to antibiotics binding the ribosome: analyses of Brucella 23S rrn, L4, L22, EF-Tu1, EF-Tu2, efflux and phylogenetic implications.BMC microbiology · 2006Article
- Mutant kinesin-2 motor subunits increase chromosome loss.Molecular biology of the cell · 2005Article
- Plus and minus sexual agglutinins from Chlamydomonas reinhardtii.The Plant cell · 2005Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
To take advantage of available expressed sequence tags and genomic sequence, we have developed 64 PCR-based molecular markers in Chlamydomonas reinhardtii that map to the 17 linkage groups. These markers will allow the rapid association of a candidate gene sequence with previously identified mutations. As proof of principle, we have identified the genes encoded by the ERY1 and ERY2 loci. Mendelian mutations that confer resistance to erythromycin define three unlinked nuclear loci in C. reinhardtii. Candidate genes ribosomal protein L4 (RPL4) and L22 (RPL22) are tightly linked to the ERY1 locus and ERY2 locus, respectively. Genomic DNA for RPL4 from wild type and five mutant ery1 alleles was amplified and sequenced and three different point mutations were found. Two different glycine residues (G(102) and G(112)) are replaced by aspartic acid and both are in the unstructured region of RPL4 that lines the peptide exit tunnel of the chloroplast ribosome. The other two alleles change a splice site acceptor site. Genomic DNA for RPL22 from wild type and three mutant ery2 alleles was amplified and sequenced and revealed three different point mutations. Two alleles have premature stop codons and one allele changes a splice site acceptor site.
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