ArticleMolecular biology of the cell2003
Grr1-dependent inactivation of Mth1 mediates glucose-induced dissociation of Rgt1 from HXT gene promoters.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers, 1 of them a synthesis that pooled it.
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Who cites it
84 citing papers in PubMed, 1 synthesis or guideline pooled it, 169 citations in OpenAlex.
- Analysing and meta-analysing time-series data of microbial growth and gene expression from plate readers.PLoS computational biology · 2022Pooled it
- Nonredundant roles for paralogous proteins in the yeast glucose-sensing pathway.Molecular biology of the cell · 2026Article
- Dynamic patterns of gene expression match extracellular signals through push-pull regulation.PLoS genetics · 2025Article
- Non-redundant roles for paralogous proteins in the yeast glucose-sensing pathway.bioRxiv : the preprint server for biology · 2025Article
- The small GTPase Rho5-Yet another player in yeast glucose signaling.PLoS genetics · 2025Article
- Functional Role of RING Ubiquitin E3 Ligase VdBre1 and VdHrd1 in the Pathogenicity and Penetration Structure Formation ofJournal of fungi (Basel, Switzerland) · 2023Article
- Review
- When yeast cells change their mind: cell cycle "Start" is reversible under starvation.The EMBO journal · 2023Article
- Investigating cell autonomy in microorganisms.Current genetics · 2022Article
- Casein kinases are required for the stability of the glucose-sensing receptor Rgt2 in yeast.Scientific reports · 2022Article
- Multi-Omics Analysis of Multiple Glucose-Sensing Receptor Systems in Yeast.Biomolecules · 2022Article
- D-Xylose Sensing inInternational journal of molecular sciences · 2021Review
- Structures and General Transport Mechanisms by the Major Facilitator Superfamily (MFS).Chemical reviews · 2021Article
- RCO-3 and COL-26 form an external-to-internal module that regulates the dual-affinity glucose transport system in Neurospora crassa.Biotechnology for biofuels · 2021Article
- More Than Just Cleaning: Ubiquitin-Mediated Proteolysis in Fungal Pathogenesis.Frontiers in cellular and infection microbiology · 2021Review
- Increased MTH1-specific 8-oxodGTPase activity is a hallmark of cancer in colon, lung and pancreatic tissue.DNA repair · 2019Article
- Genetic Analysis of Signal Generation by the Rgt2 Glucose Sensor ofG3 (Bethesda, Md.) · 2018Article
- Coordinated regulation of intracellular pH by two glucose-sensing pathways in yeast.The Journal of biological chemistry · 2018Article
- Robustness of Nutrient Signaling Is Maintained by Interconnectivity Between Signal Transduction Pathways.Frontiers in physiology · 2018Article
- Yeast phospholipase C is required for stability of casein kinase I Yck2p and expression of hexose transporters.FEMS microbiology letters · 2017Article
24 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
In budding yeast, HXT genes encoding hexose permeases are induced by glucose via a mechanism in which the F box protein Grr1 antagonizes activity of the transcriptional repressor Rgt1. Neither the mechanism of Rgt1 inactivation nor the role of Grr1 in that process has been understood. We show that glucose promotes phosphorylation of Rgt1 and its dissociation from HXT gene promoters. This cascade of events is dependent upon the F-box protein Grr1. Inactivation of Rgt1 is sufficient to explain the requirement for Grr1 but does not involve Rgt1 proteolysis or ubiquitination. We show that inactivation of Mth1 and Std1, known negative regulators of HXT gene expression, leads to the hyperphosphorylation of Rgt1 and its dissociation from HXT promoters even in the absence of glucose. Furthermore, inactivation of Mth1 and Std1 bypasses the requirement for Grr1 for induction of these events, suggesting they are targets for inactivation by Grr1. Consistent with that proposal, Mth1 is rapidly eliminated in response to glucose via a mechanism that requires Grr1. Based upon these data, we propose that glucose acts via Grr1 to promote the degradation of Mth1. Degradation of Mth1 leads to phosphorylation and dissociation of Rgt1 from HXT promoters, thereby activating HXT gene expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.