ArticleThe Journal of cell biology2003
Visualization of chromatin domains created by the gypsy insulator of Drosophila.
Article in The Journal of cell biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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Who cites it
85 citing papers in PubMed, 154 citations in OpenAlex.
- Sea CucumberResearch square · 2026Article
- Article
- Boundary stacking interactions enable cross-TAD enhancer-promoter communication during limb development.Nature genetics · 2024Article
- 3D chromatin interactions involving Drosophila insulators are infrequent but preferential and arise before TADs and transcription.Nature communications · 2023Article
- Transposable elements in mammalian chromatin organization.Nature reviews. Genetics · 2023Review
- Isha is a su(Hw) mRNA-binding protein required for gypsy insulator function.G3 (Bethesda, Md.) · 2022Article
- <i>Drosophila</i> insulator proteins exhibit in vivo liquid-liquid phase separation properties.Life science alliance · 2022Article
- Genomic organization of the autonomous regulatory domain of eyeless locus in Drosophila melanogaster.G3 (Bethesda, Md.) · 2021Article
- Plant 3D genomics: the exploration and application of chromatin organization.The New phytologist · 2021Review
- Multi-Scale Organization of theGenes · 2021Review
- Characterization of the cHS4 insulator in mouse embryonic stem cells.FEBS open bio · 2020Article
- Review
- The Role of Insulators in Transgene Transvection inGenetics · 2019Article
- The zinc-finger protein CLAMP promotesJournal of cell science · 2019Article
- The interdependence of gene-regulatory elements and the 3D genome.The Journal of cell biology · 2019Review
- Analysis of 3D genomic interactions identifies candidate host genes that transposable elements potentially regulate.Genome biology · 2018Article
- Selective interactions between diverse STEs organize the ANT-C Hox cluster.Scientific reports · 2018Article
- Double insertion of transposable elements provides a substrate for the evolution of satellite DNA.Genome research · 2018Article
- O-GlcNAcylation of boundary element associated factor (BEAF 32) in Drosophila melanogaster correlates with active histone marks at the promoters of its target genes.Nucleus (Austin, Tex.) · 2018Article
- Article
25 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Insulators might regulate gene expression by establishing and maintaining the organization of the chromatin fiber within the nucleus. Biochemical fractionation and in situ high salt extraction of lysed cells show that two known protein components of the gypsy insulator are present in the nuclear matrix. Using FISH with DNA probes located between two endogenous Su(Hw) binding sites, we show that the intervening DNA is arranged in a loop, with the two insulators located at the base. Mutations in insulator proteins, subjecting the cells to a brief heat shock, or destruction of the nuclear matrix lead to disruption of the loop. Insertion of an additional gypsy insulator in the center of the loop results in the formation of paired loops through the attachment of the inserted sequences to the nuclear matrix. These results suggest that the gypsy insulator might establish higher-order domains of chromatin structure and regulate nuclear organization by tethering the DNA to the nuclear matrix and creating chromatin loops.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.