ArticleProceedings of the National Academy of Sciences of the United States of America2003
Glutamate-binding affinity of Drosophila metabotropic glutamate receptor is modulated by association with lipid rafts.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- An octopamine-glutamate cotransmitting neuron gates aggressive escalation through layered inhibition inScience advances · 2026Article
- Lipid regulation of the glucagon receptor family.The Journal of endocrinology · 2024Review
- A cholesterol switch controls phospholipid scrambling by G protein-coupled receptors.The Journal of biological chemistry · 2024Article
- Cyclodextrins: Only Pharmaceutical Excipients or Full-Fledged Drug Candidates?Pharmaceutics · 2022Review
- The Role of Membrane Lipids in Light-Activation ofBiomolecules · 2022Review
- Cholesterol and the Safety Factor for Neuromuscular Transmission.International journal of molecular sciences · 2019Review
- Changes in the plasma membrane in metabolic disease: impact of the membrane environment on G protein-coupled receptor structure and function.British journal of pharmacology · 2018Review
- PTEN controls glandular morphogenesis through a juxtamembrane β-Arrestin1/ARHGAP21 scaffolding complex.eLife · 2017Article
- Inhibition of Ebola virus glycoprotein-mediated cytotoxicity by targeting its transmembrane domain and cholesterol.Nature communications · 2015Article
- Structure of a class C GPCR metabotropic glutamate receptor 1 bound to an allosteric modulator.Science (New York, N.Y.) · 2014Article
- Lipid rafts serve as signaling platforms for mGlu1 receptor-mediated calcium signaling in association with caveolin.Molecular brain · 2014Article
- Agonist-dependent signaling by group I metabotropic glutamate receptors is regulated by association with lipid domains.The Journal of biological chemistry · 2013Article
- Effects of palmitoylation of Cys(415) in helix 8 of the CB(1) cannabinoid receptor on membrane localization and signalling.British journal of pharmacology · 2012Article
- Sensitivity of cholecystokinin receptors to membrane cholesterol content.Frontiers in endocrinology · 2012Article
- The genetic analysis of functional connectomics in Drosophila.Advances in genetics · 2012Review
- Membrane-sensitive conformational states of helix 8 in the metabotropic Glu2 receptor, a class C GPCR.PloS one · 2012Article
- Mechanisms underlying stage-1 TRPL channel translocation in Drosophila photoreceptors.PloS one · 2012Article
- Localisation to lipid rafts correlates with increased function of the Gal/GalNAc lectin in the human protozoan parasite, Entamoeba histolytica.International journal for parasitology · 2011Article
- Specificity of intramembrane protein-lipid interactions.Cold Spring Harbor perspectives in biology · 2011Review
- Drosophila photoreceptor cells exploited for the production of eukaryotic membrane proteins: receptors, transporters and channels.PloS one · 2011Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metabotropic glutamate receptors (mGluRs) are responsible for the effects of glutamate in slow synaptic transmission, and are implicated in the regulation of many processes in the CNS. Recently, we have reported the expression and purification of a mGluR from Drosophila melanogaster (DmGluRA), a homologue of mammalian group II mGluRs. We have shown that ligand binding to reconstituted DmGluRA requires the presence of ergosterol in the liposomes [Eroglu, C., Cronet, P., Panneels, V., Beaufils, P. & Sinning, I. (2002) EMBO Rep. 3, 491-496]. Here we demonstrate that the receptor exists in different affinity states for glutamate, depending on the membrane composition. The receptor is in a high-affinity state when associated with sterol-rich lipid microdomains (rafts), and in a low-affinity state out of rafts. Enrichment of the membranes with cholesterol shifts the receptor into the high-affinity state, and induces its association with rafts. The receptor was crosslinked to photocholesterol. Our data suggest that sterol-rich lipid rafts act as positive allosteric regulators of DmGluRA.
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