ArticleRNA (New York, N.Y.)2003
Partitioning and translation of mRNAs encoding soluble proteins on membrane-bound ribosomes.
Article in RNA (New York, N.Y.), 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
80 citing papers in PubMed, 152 citations in OpenAlex.
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- Examining SRP pathway function in mRNA localization to the endoplasmic reticulum.RNA (New York, N.Y.) · 2023Article
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- Regulation of RNA localization during oocyte maturation by dynamic RNA-ER association and remodeling of the ER.Cell reports · 2022Article
- Regulation and outcomes of localized RNA translation.Wiley interdisciplinary reviews. RNA · 2022Review
- Membrane-dependent relief of translation elongation arrest on pseudouridine- and N1-methyl-pseudouridine-modified mRNAs.Nucleic acids research · 2022Article
- HDLBP binds ER-targeted mRNAs by multivalent interactions to promote protein synthesis of transmembrane and secreted proteins.Nature communications · 2022Article
- Recruitment of endoplasmic reticulum-targeted and cytosolic mRNAs into membrane-associated stress granules.RNA (New York, N.Y.) · 2021Article
- Review
- mRNA Targeting, Transport and Local Translation in Eukaryotic Cells: From the Classical View to a Diversity of New Concepts.Molecular biology · 2021Article
- Quantitative Proteomics Links the LRRC59 Interactome to mRNA Translation on the ER Membrane.Molecular & cellular proteomics : MCP · 2020Article
- Comparison of RNA isolation methods on RNA-Seq: implications for differential expression and meta-analyses.BMC genomics · 2020Article
- A secretion-enhancing cis regulatory targeting element (SECReTE) involved in mRNA localization and protein synthesis.PLoS genetics · 2019Article
- Phosphorylation-dependent Regnase-1 release from endoplasmic reticulum is critical in IL-17 response.The Journal of experimental medicine · 2019Article
- Component of splicing factor SF3b plays a key role in translational control of polyribosomes on the endoplasmic reticulum.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Activation of the DNA-repair mechanism through NBS1 and MRE11 diffusion.PLoS computational biology · 2018Article
- Oncoprotein AEG-1 is an endoplasmic reticulum RNA-binding protein whose interactome is enriched in organelle resident protein-encoding mRNAs.RNA (New York, N.Y.) · 2018Article
- Simple paired heavy- and light-chain antibody repertoire sequencing using endoplasmic reticulum microsomes.Genome medicine · 2018Article
20 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
In eukaryotic cells, it is generally accepted that protein synthesis is compartmentalized; soluble proteins are synthesized on free ribosomes, whereas secretory and membrane proteins are synthesized on endoplasmic reticulum (ER)-bound ribosomes. The partitioning of mRNAs that accompanies such compartmentalization arises early in protein synthesis, when ribosomes engaged in the translation of mRNAs encoding signal-sequence-bearing proteins are targeted to the ER. In this report, we use multiple cell fractionation protocols, in combination with cDNA microarray, nuclease protection, and Northern blot analyses, to assess the distribution of mRNAs between free and ER-bound ribosomes. We find a broad representation of mRNAs encoding soluble proteins in the ER fraction, with a subset of such mRNAs displaying substantial ER partitioning. In addition, we present evidence that membrane-bound ribosomes engage in the translation of mRNAs encoding soluble proteins. Single-cell in situ hybridization analysis of the subcellular distribution of mRNAs encoding ER-localized and soluble proteins identify two overall patterns of mRNA distribution in the cell-endoplasmic reticular and cytosolic. However, both partitioning patterns include a distinct perinuclear component. These results identify previously unappreciated roles for membrane-bound ribosomes in the subcellular compartmentalization of protein synthesis and indicate possible functions for the perinuclear membrane domain in mRNA sorting in the cell.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.