ArticleRNA (New York, N.Y.)2003
Localization of nuclear retained mRNAs in Saccharomyces cerevisiae.
Article in RNA (New York, N.Y.), 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 68 citations in OpenAlex.
- Nuclear mRNA degradation tunes the gain of the unfolded protein response in Saccharomyces cerevisiae.Nucleic acids research · 2018Article
- Review
- mRNA quality control is bypassed for immediate export of stress-responsive transcripts.Nature · 2016Article
- Altered RNA processing and export lead to retention of mRNAs near transcription sites and nuclear pore complexes or within the nucleolus.Molecular biology of the cell · 2016Article
- Meiotic long non-coding meiRNA accumulates as a dot at its genetic locus facilitated by Mmi1 and plays as a decoy to lure Mmi1.Open biology · 2014Article
- Mutational analysis of the yeast RNA helicase Sub2p reveals conserved domains required for growth, mRNA export, and genomic stability.RNA (New York, N.Y.) · 2013Article
- RNA degradation in Saccharomyces cerevisae.Genetics · 2012Review
- Implication of Ccr4-Not complex function in mRNA quality control in Saccharomyces cerevisiae.RNA (New York, N.Y.) · 2011Article
- A nucleoporin, Nup60p, affects the nuclear and cytoplasmic localization of ASH1 mRNA in S. cerevisiae.RNA (New York, N.Y.) · 2011Article
- A link between nuclear RNA surveillance, the human exosome and RNA polymerase II transcriptional termination.Nucleic acids research · 2010Article
- Assembly of an export-competent mRNP is needed for efficient release of the 3'-end processing complex after polyadenylation.Molecular and cellular biology · 2009Article
- Core exosome-independent roles for Rrp6 in cell cycle progression.Molecular biology of the cell · 2009Article
- Exonucleolysis is required for nuclear mRNA quality control in yeast THO mutants.RNA (New York, N.Y.) · 2008Article
- The T body, a new cytoplasmic RNA granule in Saccharomyces cerevisiae.Molecular and cellular biology · 2008Article
- Nuclear transit of the RNA-binding protein She2 is required for translational control of localized ASH1 mRNA.EMBO reports · 2008Article
- General, rapid, and transcription-dependent fragmentation of nucleolar antigens in S. cerevisiae mRNA export mutants.RNA (New York, N.Y.) · 2008Article
- The exosome and RNA quality control in the nucleus.EMBO reports · 2007Review
- Dissecting mechanisms of nuclear mRNA surveillance in THO/sub2 complex mutants.The EMBO journal · 2007Article
- The PMC2NT domain of the catalytic exosome subunit Rrp6p provides the interface for binding with its cofactor Rrp47p, a nucleic acid-binding protein.Nucleic acids research · 2007Article
- 3'-end formation signals modulate the association of genes with the nuclear periphery as well as mRNP dot formation.The EMBO journal · 2006Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the yeast Saccharomyces cerevisiae, a common conditional phenotype associated with deletion or mutation of genes encoding mRNA export factors is the rapid accumulation of mRNAs in intranuclear foci, suggested to be near transcription sites. The nuclear RNA exosome has been implicated in retaining RNAs in these foci; on deletion of the exosome component Rrp6p, the RNA is released. To determine the exact nuclear location of retained as well as released mRNAs, we have used mRNA export mutant strains to analyze the spatial relationship between newly synthesized heat shock mRNA, the chromosomal site of transcription, and known S. cerevisiae nuclear structures such as the nucleolus and the nucleolar body. Our results show that retained SSA4 RNA localizes to an area in close proximity to the SSA4 locus. On deletion of Rrp6p and release from the genomic locus, heat shock mRNAs produced in the rat7-1 strain colocalize predominantly with nucleolar antigens. Bulk poly(A)(+) RNA, on the other hand, is localized primarily to the nuclear rim. Interestingly, the RNA binding nucleocytoplasmic shuttle protein Npl3p shows strong colocalization with bulk poly(A)(+) RNA, regardless of its nuclear location. Taken together, our data show that retention occurs close to the gene and indicate distinct nuclear fates of different mRNAs.
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Registered trials
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