Evidence map›Paper›PMID 12923096›Full record

ArticleJournal of bacteriology2003

Topology of RbsC, the membrane component of the Escherichia coli ribose transporter.

Jeffrey B Stewart, Mark A Hermodson

Abstract read
In one paragraph

Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Contact-dependent growth inhibition toxins exploit multiple independent cell-entry pathways.Proceedings of the National Academy of Sciences of the United States of America · 2015
    Article
  6. Article
  7. Article
  8. ABC transporters: the power to change.Nature reviews. Molecular cell biology · 2009
    Review
  9. Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Jeffrey B StewartDepartment of Biochemistry, Purdue University, 175 S. University Street, West Lafayette, IN 49707, USA.
Mark A Hermodson

Funding

BIOPHYS STUD OF PROTEINS, NUCLEIC ACIDS, AND VIRUSEST32GM008296 · NIGMS · PURDUE UNIVERSITY WEST LAFAYETTE · PI STAUFFACHER, CYNTHIA VIANNE · 1989 to 2012
$2.2M
STRUCTURE OF PROTEINS OF THE RIBOSE TRANSPORT SYSTEMR01GM054993 · NIGMS · PURDUE UNIVERSITY WEST LAFAYETTE · PI HERMODSON, MARK A · 1997 to 2000
$270k
NIGMS NIH HHS GM 54993NIGMS NIH HHS T32 GM008296NIGMS NIH HHS T32 GM 08296
6 · The paper itself

Abstract

The topology of RbsC, the membrane component of the ribose transporter in Escherichia coli, has been determined by using 34 single-cysteine mutants and a modified fluorescence labeling technique designated multiplex labeling. This technique gives topology, expression, and localization information for a membrane protein from a single batch of bacterial cells. The results indicate that RbsC contains 10 transmembrane-spanning helices, with the N and C termini being in the cytosol. This topology matches predictions from the latest prediction programs and the topology of the similar, recently crystallized membrane protein BtuC.

Indexed as

Amino Acid SequenceAmino Acid SubstitutionATP-Binding Cassette TransportersBiological TransportCell MembraneCysteineEscherichia coliEscherichia coli ProteinsFluoresceinModels, MolecularMolecular Sequence DataMutagenesis, Site-DirectedMutationRiboseATP-Binding Cassette TransportersCysteineEscherichia coli ProteinsFluoresceinRbsC protein, E coliRibose

Identifiers

PMID12923096
PMCPMC181013

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.