ArticleGenes & development2003
Association of the RENT complex with nontranscribed and coding regions of rDNA and a regional requirement for the replication fork block protein Fob1 in rDNA silencing.
Article in Genes & development, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 143 papers.
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Who cites it
143 citing papers in PubMed.
- ERC accumulation depletes Sir2 from rDNA and induces cellular senescence by rDNA destabilization.Nucleic acids research · 2025Article
- Article
- Transcription near arrested DNA replication forks triggers ribosomal DNA copy number changes.Nucleic acids research · 2025Article
- Sir2 is required for the quiescence-specific condensed three-dimensional chromatin structure of rDNA.bioRxiv : the preprint server for biology · 2024Article
- Non-canonical chromatin-based functions for the threonine metabolic pathway.Scientific reports · 2024Article
- Saccharomyces cerevisiae deficient in the early anaphase release of Cdc14 can traverse anaphase I without ribosomal DNA disjunction and successfully complete meiosis.Biology open · 2023Article
- Broadly Applicable Control Approaches Improve Accuracy of ChIP-Seq Data.International journal of molecular sciences · 2023Article
- Fob1-dependent condensin recruitment and loop extrusion on yeast chromosome III.PLoS genetics · 2023Article
- Ribosomal DNA replication time coordinates completion of genome replication and anaphase in yeast.Cell reports · 2023Article
- SWR1 chromatin remodeling complex prevents mitotic slippage during spindle position checkpoint arrest.Molecular biology of the cell · 2023Article
- Spreading-dependent or independent Sir2-mediated gene silencing in budding yeast.Genes & genomics · 2022Article
- The regional sequestration of heterochromatin structural proteins is critical to form and maintain silent chromatin.Epigenetics & chromatin · 2022Review
- Smc5/6 in the rDNA modulates lifespan independently of Fob1.Aging cell · 2021Article
- The rDNA is biomolecular condensate formed by polymer-polymer phase separation and is sequestered in the nucleolus by transcription and R-loops.Nucleic acids research · 2021Article
- The trehalose-6-phosphate phosphatase Tps2 regulatesAutophagy · 2021Article
- Exonuclease resistant 18S and 25S ribosomal RNA components in yeast are possibly newly transcribed by RNA polymerase II.BMC molecular and cell biology · 2020Article
- TORC1 signaling regulates cytoplasmic pH through Sir2 in yeast.Aging cell · 2020Article
- Yeast mismatch repair components are required for stable inheritance of gene silencing.PLoS genetics · 2020Article
- Structural basis of RNA polymerase I pre-initiation complex formation and promoter melting.Nature communications · 2020Article
- Long-range single-molecule mapping of chromatin accessibility in eukaryotes.Nature methods · 2020Article
83 more citing papers are in PubMed but not listed here.
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2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Silencing within the yeast rDNA repeats inhibits hyperrecombination, represses transcription from foreign promoters, and extends replicative life span. rDNA silencing is mediated by a Sir2-containing complex called RENT (regulator of nucleolar silencing and telophase exit). We show that the Net1 (also called Cfi1) and Sir2 subunits of RENT localize primarily to two distinct regions within rDNA: in one of the nontranscribed spacers (NTS1) and around the Pol I promoter, extending into the 35S rRNA coding region. Binding to NTS1 overlaps the recombination hotspot and replication fork barrier elements, which have been shown previously to require the Fob1 protein for their activities. In cells lacking Fob1, silencing and the association of RENT subunits are abolished specifically at NTS1, while silencing and association at the Pol I promoter region are unaffected or increased. We find that Net1 and Sir2 are physically associated with Fob1 and subunits of RNA polymerase I. Together with the localization data, these results suggest the existence of two distinct modes for the recruitment of the RENT complex to rDNA and reveal a role for Fob1 in rDNA silencing and in the recruitment of the RENT complex. Furthermore, the Fob1-dependent associations of Net1 and Sir2 with the recombination hotspot region strongly suggest that Sir2 acts directly at this region to carry out its inhibitory effect on rDNA recombination and accelerated aging.
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