ArticleGenes & development2003
DNA binding controls inactivation and nuclear accumulation of the transcription factor Stat1.
Article in Genes & development, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
64 citing papers in PubMed, 159 citations in OpenAlex.
- Inborn errors of immunity with loss- and gain-of-function germline mutations in STAT1.Clinical and experimental immunology · 2023Review
- Lack of STAT1 co-operative DNA binding protects against adverse cardiac remodelling in acute myocardial infarction.Frontiers in cardiovascular medicine · 2023Article
- An inhibitory effect on the nuclear accumulation of phospho-STAT1 by its unphosphorylated form.Cell communication and signaling : CCS · 2022Article
- Transcriptional inhibition of STAT1 functions in the nucleus alleviates Th1 and Th17 cell-mediated inflammatory diseases.Frontiers in immunology · 2022Article
- STAT proteins: a kaleidoscope of canonical and non-canonical functions in immunity and cancer.Journal of hematology & oncology · 2021Review
- Phospho-Ser727 triggers a multistep inactivation of STAT3 by rapid dissociation of pY705-SH2 through C-terminal tail modulation.International immunology · 2020Article
- Review
- Crosstalk between type I and II interferons in regulation of myeloid cell responses during bacterial infection.Current opinion in immunology · 2018Review
- Cucurbitacin I inhibits STAT3, but enhances STAT1 signaling in human cancer cells in vitro through disrupting actin filaments.Acta pharmacologica Sinica · 2018Article
- Nuclear RNF2 inhibits interferon function by promoting K33-linked STAT1 disassociation from DNA.Nature immunology · 2018Article
- A Positive Feedback Amplifier Circuit That Regulates Interferon (IFN)-Stimulated Gene Expression and Controls Type I and Type II IFN Responses.Frontiers in immunology · 2018Review
- Properties of STAT1 and IRF1 enhancers and the influence of SNPs.BMC molecular biology · 2017Article
- Overexpression of Semaphorin-3E enhances pancreatic cancer cell growth and associates with poor patient survival.Oncotarget · 2016Article
- Cystatin B and HIV regulate the STAT-1 signaling circuit in HIV-infected and INF-β-treated human macrophages.Journal of neurovirology · 2016Article
- Go in! Go out! Inducible control of nuclear localization.Current opinion in chemical biology · 2016Review
- VP8, the Major Tegument Protein of Bovine Herpesvirus 1, Interacts with Cellular STAT1 and Inhibits Interferon Beta Signaling.Journal of virology · 2016Article
- NLRP3 tyrosine phosphorylation is controlled by protein tyrosine phosphatase PTPN22.The Journal of clinical investigation · 2016Article
- Novel drug targets for personalized precision medicine in relapsed/refractory diffuse large B-cell lymphoma: a comprehensive review.Molecular cancer · 2015Review
- Mutations in the linker domain affect phospho-STAT3 function and suggest targets for interrupting STAT3 activity.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Promoter occupancy of STAT1 in interferon responses is regulated by processive transcription.Molecular and cellular biology · 2015Article
4 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytokine-dependent gene transcription greatly depends on the tyrosine phosphorylation ("activation") of Stat proteins at the cell membrane. This rapidly leads to their accumulation in the nucleus by an unknown mechanism. We performed microinjections of recombinant Stat1 protein to show that nuclear accumulation of phosphorylated Stat1 can occur without cytokine stimulation of cells. Microinjection of Stat1 antibody and treatment of cells with kinase or phosphatase inhibitors revealed that nuclear accumulation is a highly dynamic process sustained by Stat1 nucleocytoplasmic cycling and continuous kinase activity. By characterizing nuclear accumulation mutants, it is demonstrated that nuclear import and nuclear retention are two separate steps leading up to nuclear accumulation, with nonspecific DNA binding of activated Stat1 being sufficient for nuclear retention. Critical for nuclear buildup of Stat1 and the subsequent nuclear export is the point of time of tyrosine dephosphorylation, because our data indicate that activated Stat1 is incapable of leaving the nucleus and requires dephosphorylation to do so. It is demonstrated that the inactivation of Stat1 is controlled by its exchange reaction with DNA, whereby DNA binding protects Stat1 from dephosphorylation in a sequence-specific manner. Thus, during nuclear accumulation, a surprisingly simple mechanism integrates central aspects of cytokine-dependent gene regulation, for example, receptor monitoring, promoter occupancy, and transcription factor inactivation.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.