Evidence map›Paper›PMID 12917416›Full record

ArticleThe Journal of biological chemistry2003

Mouse Snail family transcription repressors regulate chondrocyte, extracellular matrix, type II collagen, and aggrecan.

Kenji Seki, Toshihiko Fujimori, Pierre Savagner, Akiko Hata, Tomonao Aikawa, Naoshi Ogata, Yoichi Nabeshima, Lee Kaechoong

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
1.7field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed, 85 citations in OpenAlex.

  1. Review
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  13. Achondroplasia: Development, pathogenesis, and therapy.Developmental dynamics : an official publication of the American Association of Anatomists · 2017
    Review
  14. Article
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  19. Compensatory regulation of the Snai1 and Snai2 genes during chondrogenesis.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2013
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 3 countries.

Kenji SekiEndocrine Unit, Massachusetts General Hospital, Boston, MA 02114, USA. kseki@anat1.med.kyoto-u.ac.jp
Toshihiko Fujimori
Pierre Savagner
Akiko Hata
Tomonao Aikawa
Naoshi Ogata
Yoichi Nabeshima
Lee Kaechoong
Kyoto University · JPMassachusetts General Hospital · USInserm · FRTufts Medical Center · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Snail family genes are conserved among species during evolution and encode transcription factors expressed at different stages of development in different tissues. These genes are involved in a broad spectrum of biological functions: cell differentiation, cell motility, cell cycle regulation, and apoptosis. However, little is known about the target genes involved in these functions. Here we show that mouse Snail family members, Snail (Sna) and Slug (Slugh), are involved in chondrocyte differentiation by controlling the expression of type II collagen (Col2a1) and aggrecan. In situ hybridization analysis of developing mouse limb demonstrated that Snail and Slug mRNAs were highly expressed in hypertrophic chondrocytes. Inversely, the expression of collagen type II mRNA disappeared during hypertrophic differentiation. Snail and Slug mRNA expression was down-regulated during differentiation of the mouse chondrogenic cell line ATDC5 and overexpression of exogenous Snail or Slug in ATDC5 cells inhibited expression of collagen type II and aggrecan mRNA. Reporter analysis revealed Snail and Slug suppressed the promoter activity of Col2a1, and the E-boxes in the promoter region were the responsible element. Gel shift assay demonstrated the binding of Snail to the E-box. Because type II collagen and aggrecan are major functional components of extracellular matrix in cartilage, these results suggest an important role for Snail-related transcription repressors during chondrocyte differentiation.

Indexed as

Extracellular Matrix ProteinsAggrecansAnimalsCell DifferentiationCell LineChondrocytesCollagen Type IIDNA-Binding ProteinsE-Box ElementsEmbryo, MammalianExtracellular MatrixGene Expression Regulation, DevelopmentalLectins, C-TypeMicePromoter Regions, GeneticProteoglycansAcan protein, mouseAggrecansCollagen Type IIDNA-Binding ProteinsExtracellular Matrix ProteinsLectins, C-TypeProteoglycansRepressor ProteinsRNA, MessengerSnai2 protein, mouseSnail Family Transcription FactorsTranscription Factors

Identifiers

PMID12917416
PMCPMC2253659
OpenAlexW2035474317

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.