ArticleMolecular and cellular biology2003
Rad53 phosphorylation site clusters are important for Rad53 regulation and signaling.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 106 citations in OpenAlex.
- Comprehensive analysis of the functional impact of single nucleotide variants of human CHEK2.PLoS genetics · 2024Article
- Rad53 regulates the lifetime of Rdh54 at homologous recombination intermediates.Nucleic acids research · 2023Article
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- Review
- The DNA damage checkpoint: A tale from budding yeast.Frontiers in genetics · 2022Review
- The Effect of Dia2 Protein Deficiency on the Cell Cycle, Cell Size, and Recruitment of Ctf4 Protein inMolecules (Basel, Switzerland) · 2021Article
- DNA damage response of major fungal pathogen Candida glabrata offers clues to explain its genetic diversity.Current genetics · 2021Review
- Humanizing the yeast origin recognition complex.Nature communications · 2021Article
- Article
- Dual roles of yeast Rad51 N-terminal domain in repairing DNA double-strand breaks.Nucleic acids research · 2020Article
- Tel1 Activation by the MRX Complex Is Sufficient for Telomere Length Regulation but Not for the DNA Damage Response inGenetics · 2019Article
- Activation of Tel1The Journal of biological chemistry · 2019Article
- PP2A Controls Genome Integrity by Integrating Nutrient-Sensing and Metabolic Pathways with the DNA Damage Response.Molecular cell · 2017Article
- Yeast Dun1 Kinase Regulates Ribonucleotide Reductase Small Subunit Localization in Response to Iron Deficiency.The Journal of biological chemistry · 2016Article
- Requirement of the FATC domain of protein kinase Tel1 for localization to DNA ends and target protein recognition.Molecular biology of the cell · 2015Article
- DNA Damage Response Checkpoint Activation Drives KP1019 Dependent Pre-Anaphase Cell Cycle Delay in S. cerevisiae.PloS one · 2015Article
- Yeast Dun1 kinase regulates ribonucleotide reductase inhibitor Sml1 in response to iron deficiency.Molecular and cellular biology · 2014Article
- Use of quantitative mass spectrometric analysis to elucidate the mechanisms of phospho-priming and auto-activation of the checkpoint kinase Rad53 in vivo.Molecular & cellular proteomics : MCP · 2014Article
- Crosstalk between mitochondrial stress signals regulates yeast chronological lifespan.Mechanisms of ageing and development · 2014Article
- Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Budding yeast Rad53 is an essential protein kinase that is phosphorylated and activated in a MEC1- and TEL1-dependent manner in response to DNA damage. We studied the role of Rad53 phosphorylation through mutation of consensus phosphorylation sites for upstream kinases Mec1 and Tel1. Alanine substitution of the Rad53 amino-terminal TQ cluster region reduced viability and impaired checkpoint functions. These substitution mutations spared the basal interaction with Asf1 and the DNA damage-induced interactions with Rad9. However, they caused a decrease in DNA damage-induced Rad53 kinase activity and an impaired interaction with the protein kinase Dun1. The Dun1 FHA (Forkhead-associated) domain recognized the amino-terminal TQ cluster of Rad53 after DNA damage or replication blockade. Thus, the phosphorylation of Rad53 by upstream kinases is important not only for Rad53 activation but also for creation of an interface between Rad53 and Dun1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.