ArticleJournal of virology2003
Susceptibility of human hepatitis delta virus RNAs to small interfering RNA action.
Article in Journal of virology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 60 citations in OpenAlex.
- Sensitive reverse transcription-digital droplet PCR-based discrimination of hepatitis delta virus RNAs reveals differential effects of interferon-α on viral RNA species.The Journal of general virology · 2026Article
- Review
- Targeting HBV with RNA interference: Paths to cure.Science translational medicine · 2025Review
- Identification of selective hepatitis delta virus ribozyme inhibitors by high-throughput screening of small molecule libraries.JHEP reports : innovation in hepatology · 2023Article
- Review article: emerging insights into the immunopathology, clinical and therapeutic aspects of hepatitis delta virus.Alimentary pharmacology & therapeutics · 2022Review
- Inspecting the Ribozyme Region of Hepatitis Delta Virus Genotype 1: Conservation and Variability.Viruses · 2022Article
- Review
- Hepatitis delta virus: insights into a peculiar pathogen and novel treatment options.Nature reviews. Gastroenterology & hepatology · 2016Review
- Animal models of chronic hepatitis delta virus infection host-virus immunologic interactions.Pathogens (Basel, Switzerland) · 2015Review
- Design of potential siRNA molecules for hepatitis delta virus gene silencing.Bioinformation · 2012Article
- Article
- Combined proteomic-RNAi screen for host factors involved in human hepatitis delta virus replication.RNA (New York, N.Y.) · 2009Article
- Interfering RNA and HIV: reciprocal interferences.PLoS pathogens · 2008Review
- Hepatitis d.Current treatment options in gastroenterology · 2007Article
- RNA interference against viruses: strike and counterstrike.Nature biotechnology · 2007Review
- Assembly of hepatitis B virus envelope proteins onto a lentivirus pseudotype that infects primary human hepatocytes.Journal of virology · 2007Article
- RNA interference for antiviral therapy.The journal of gene medicine · 2006Review
- The interplay between virus infection and the cellular RNA interference machinery.FEBS letters · 2006Review
- RNA interference: its use as antiviral therapy.Handbook of experimental pharmacology · 2006Review
- Development of resistance to RNAi in mammalian cells.Annals of the New York Academy of Sciences · 2005Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
In animal cells, small interfering RNAs (siRNA), when exogenously provided, have been reported to be capable of inhibiting replication of several different viruses. In preliminary studies, siRNA species were designed and tested for their ability to act on the protein expressed in Huh7 cells transfected with DNA-directed mRNA constructs containing hepatitis delta virus (HDV) target sequences. The aim was to achieve siRNA specific for each of the three RNAs of HDV replication: (i) the 1,679-nucleotide circular RNA genome, (ii) its exact complement, the antigenome, and (iii) the less abundant polyadenylated mRNA for the small delta protein. Many of the 16 siRNA tested gave >80% inhibition in this assay. Next, these three classes of siRNA were tested for their ability to act during HDV genome replication. It was found that only siRNA targeted against HDV mRNA sequences could interfere with HDV genome replication. In contrast, siRNA targeted against genomic and antigenomic RNA sequences had no detectable effect on the accumulation of these RNAs. Reconstruction experiments with nonreplicating HDV RNA sequences support the interpretation that neither the potential for intramolecular rod-like RNA folding nor the presence of the delta protein conferred resistance to siRNA. In terms of replicating HDV RNAs, it is considered more likely that the genomic and antigenomic RNAs are resistant because their location within the nucleus makes them inaccessible to siRNA-mediated degradation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.