ArticlePlant physiology2003
The Chlamydomonas reinhardtii cia3 mutant lacking a thylakoid lumen-localized carbonic anhydrase is limited by CO2 supply to rubisco and not photosystem II function in vivo.
Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 87 citations in OpenAlex.
- Rapid reconstitution and kinase-controlled regulation of algal pyrenoid condensates in engineeredbioRxiv : the preprint server for biology · 2026Article
- Sensitivity of Photosystem II to Photoinhibition inInternational journal of molecular sciences · 2026Article
- Super-resolution expansion microscopy reveals nanoscale protein domains and CObioRxiv : the preprint server for biology · 2026Article
- CAH3 fromCells · 2024Review
- The pyrenoid: the eukaryotic CO2-concentrating organelle.The Plant cell · 2023Article
- Adapting from Low to High: An Update to COPlants (Basel, Switzerland) · 2023Review
- A Rapid Method for Detecting Normal or Modified Plant and Algal Carbonic Anhydrase Activity UsingPlants (Basel, Switzerland) · 2022Article
- Modelling the pyrenoid-based CONature plants · 2022Article
- The Main Structural and Functional Characteristics of Photosystem-II-Enriched Membranes Isolated from Wild Type andLife (Basel, Switzerland) · 2020Article
- Membrane Inlet Mass Spectrometry: A Powerful Tool for Algal Research.Frontiers in plant science · 2020Article
- An Update on the Metabolic Roles of Carbonic Anhydrases in the Model Alga Chlamydomonas reinhardtii.Metabolites · 2018Review
- Plant Carbonic Anhydrases: Structures, Locations, Evolution, and Physiological Roles.Molecular plant · 2017Review
- Structural insights into the LCIB protein family reveals a new group of β-carbonic anhydrases.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Metabolomic Responses of Arabidopsis Suspension Cells to Bicarbonate under Light and Dark Conditions.Scientific reports · 2016Article
- Native architecture of the Chlamydomonas chloroplast revealed by in situ cryo-electron tomography.eLife · 2015Article
- Control of Rubisco function via homeostatic equilibration of CO2 supply.Frontiers in plant science · 2015Article
- LCIB in the Chlamydomonas CO2-concentrating mechanism.Photosynthesis research · 2014Article
- The carbon concentrating mechanism in Chlamydomonas reinhardtii: finding the missing pieces.Photosynthesis research · 2014Article
- Comparing the in vivo function of α-carboxysomes and β-carboxysomes in two model cyanobacteria.Plant physiology · 2014Article
- Mobile hydrogen carbonate acts as proton acceptor in photosynthetic water oxidation.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Chlamydomonas reinhardtii cia3 mutant has a phenotype indicating that it requires high-CO(2) levels for effective photosynthesis and growth. It was initially proposed that this mutant was defective in a carbonic anhydrase (CA) that was a key component of the photosynthetic CO(2)-concentrating mechanism (CCM). However, more recent identification of the genetic lesion as a defect in a lumenal CA associated with photosystem II (PSII) has raised questions about the role of this CA in either the CCM or PSII function. To resolve the role of this lumenal CA, we re-examined the physiology of the cia3 mutant. We confirmed and extended previous gas exchange analyses by using membrane-inlet mass spectrometry to monitor(16)O(2),(18)O(2), and CO(2) fluxes in vivo. The results demonstrate that PSII electron transport is not limited in the cia3 mutant at low inorganic carbon (Ci). We also measured metabolite pools sizes and showed that the RuBP pool does not fall to abnormally low levels at low Ci as might be expected by a photosynthetic electron transport or ATP generation limitation. Overall, the results demonstrate that under low Ci conditions, the mutant lacks the ability to supply Rubisco with adequate CO(2) for effective CO(2) fixation and is not limited directly by any aspect of PSII function. We conclude that the thylakoid CA is primarily required for the proper functioning of the CCM at low Ci by providing an ample supply of CO(2) for Rubisco.
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