ArticlePlant physiology2003
Three nicotianamine synthase genes isolated from maize are differentially regulated by iron nutritional status.
Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 119 citations in OpenAlex.
- OsFPN1 functions as an essential metal transporter for Fe homeostasis and Co/Ni tolerance in rice.Plant molecular biology · 2026Article
- Expanding Iron Acquisition in Maize: Root Sector-Specific Responses and Gibberellin Regulation of Ferric and Ferrous Iron Uptake.International journal of molecular sciences · 2026Article
- Identification of the Nicotianamine synthase (NAS) gene family in wheat (Triticum aestivum L.) and the role of its member TaNAS4-A in Zn and Fe transport.Functional & integrative genomics · 2025Article
- Barriers and carriers for transition metal homeostasis in plants.Plant communications · 2025Review
- Molecular-based characterization and bioengineering of Sorghum bicolor to enhance iron deficiency tolerance in iron-limiting calcareous soils.Plant molecular biology · 2024Article
- Zeocin-induced DNA damage response in barley and its dependence on ATR.Scientific reports · 2024Article
- Nicotianamine: A Key Player in Metal Homeostasis and Hyperaccumulation in Plants.International journal of molecular sciences · 2023Review
- Brassinosteroids and gibberellic acid actively regulate the zinc detoxification mechanism of Medicago sativa L. seedlings.BMC plant biology · 2023Article
- Exploring key developmental phases and phase-specific genes across the entirety of anther development in maize.Journal of integrative plant biology · 2022Article
- Comparative Transcriptome Analysis of Iron and Zinc Deficiency in Maize (Plants (Basel, Switzerland) · 2020Article
- Discovery and validation of candidate genes for grain iron and zinc metabolism in pearl millet [Pennisetum glaucum (L.) R. Br.].Scientific reports · 2020Article
- Potential Implications of Interactions between Fe and S on Cereal Fe Biofortification.International journal of molecular sciences · 2020Review
- Nicotianamine Synthesis byFrontiers in plant science · 2019Article
- Article
- Molecular Profiling of Pierce's Disease Outlines the Response Circuitry ofFrontiers in plant science · 2018Article
- Investigation of Baseline Iron Levels in Australian Chickpea and Evaluation of a Transgenic Biofortification Approach.Frontiers in plant science · 2018Article
- The Nicotianamine Synthase Gene Is a Useful Candidate for Improving the Nutritional Qualities and Fe-Deficiency Tolerance of Various Crops.Frontiers in plant science · 2018Review
- Identification and molecular characterization of the nicotianamine synthase gene family in bread wheat.Plant biotechnology journal · 2016Article
- The Phytosiderophore Efflux Transporter TOM2 Is Involved in Metal Transport in Rice.The Journal of biological chemistry · 2015Article
- Arbuscular mycorrhizal symbiosis alters the expression patterns of three key iron homeostasis genes, ZmNAS1, ZmNAS3, and ZmYS1, in S deprived maize plants.Frontiers in plant science · 2015Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nicotianamine synthase (NAS) is an enzyme that is critical for the biosynthesis of the mugineic acid family of phytosiderophores in graminaceous plants, and for the homeostasis of metal ions in nongraminaceous plants. We isolated one genomic NAS clone, ZmNAS3, and two cDNA NAS clones, ZmNAS1 and ZmNAS2, from maize (Zea mays cv Alice). In agreement with the increased secretion of phytosiderophores with Fe deficiency, ZmNAS1 and ZmNAS2 were positively expressed only in Fe-deficient roots. In contrast, ZmNAS3 was expressed under Fe-sufficient conditions, and was negatively regulated by Fe deficiency. This is the first report describing down-regulation of NAS gene expression in response to Fe deficiency in plants, shedding light on the role of nicotianamine in graminaceous plants, other than as a precursor in phytosiderophore production. ZmNAS1-green fluorescent protein (sGFP) and ZmNAS2-sGFP were localized at spots in the cytoplasm of onion (Allium cepa) epidermal cells, whereas ZmNAS3-sGFP was distributed throughout the cytoplasm of these cells. ZmNAS1 and ZmNAS3 showed NAS activity in vitro, whereas ZmNAS2 showed none. Due to its duplicated structure, ZmNAS2 was much larger (65.8 kD) than ZmNAS1, ZmNAS3, and previously characterized NAS proteins (30-38 kD) from other plant species. We reveal that maize has two types of NAS proteins based on their expression pattern and subcellular localization.
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