ArticlePlant physiology2003
Structure and properties of an engineered transketolase from maize.
Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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Who cites it
14 citing papers in PubMed, 52 citations in OpenAlex.
- Investigations into Simplified Analogues of the Herbicidal Natural Product (+)-Cornexistin.Chemistry (Weinheim an der Bergstrasse, Germany) · 2023Article
- A 'Split-Gene' Transketolase From the Hyper-Thermophilic BacteriumFrontiers in microbiology · 2020Article
- Structure-Based Discovery and Synthesis of Potential Transketolase Inhibitors.Molecules (Basel, Switzerland) · 2018Article
- High-resolution structures of Lactobacillus salivarius transketolase in the presence and absence of thiamine pyrophosphate.Acta crystallographica. Section F, Structural biology communications · 2015Article
- Phosphorylation of Arabidopsis transketolase at Ser428 provides a potential paradigm for the metabolic control of chloroplast carbon metabolism.The Biochemical journal · 2014Article
- Cloning, expression and characterization of sugarcane (Saccharum officinarum L.) transketolase.The protein journal · 2013Article
- Article
- Proteomic analysis revealed nitrogen-mediated metabolic, developmental, and hormonal regulation of maize (Zea mays L.) ear growth.Journal of experimental botany · 2012Article
- Diphenyl urea derivatives as inhibitors of transketolase: a structure-based virtual screening.PloS one · 2012Article
- Structure and function of the transketolase from Mycobacterium tuberculosis and comparison with the human enzyme.Open biology · 2012Article
- The crystal structure of human transketolase and new insights into its mode of action.The Journal of biological chemistry · 2010Article
- Article
- Structure-activity relationships of compounds targeting mycobacterium tuberculosis 1-deoxy-D-xylulose 5-phosphate synthase.Bioorganic & medicinal chemistry letters · 2008Article
- Transketolase from Leishmania mexicana has a dual subcellular localization.The Biochemical journal · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gene specifying plastid transketolase (TK) of maize (Zea mays) was cloned from a cDNA library by southern blotting using a heterologous probe from sorghum (Sorghum bicolor). A recombinant fusion protein comprising thioredoxin of Escherichia coli and mature TK of maize was expressed at a high level in E. coli and cleaved with thrombin, affording plastid TK. The protein in complex with thiamine pyrophoshate was crystallized, and its structure was solved by molecular replacement. The enzyme is a C2 symmetric homodimer closely similar to the enzyme from yeast (Saccharomyces cerevisiae). Each subunit is folded into three domains. The two topologically equivalent active sites are located in the subunit interface region and resemble those of the yeast enzyme.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.