ArticlePlant physiology2003
Subcellular targeting of nine calcium-dependent protein kinase isoforms from Arabidopsis.
Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 106 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
106 citing papers in PubMed, 259 citations in OpenAlex.
- Calcium-dependent protein kinase signalling in plant immune responses: current status and future prospects.Planta · 2026Review
- Natural variation inScience advances · 2026Article
- Peroxisome calcium uptake is dependent on ER-peroxisome membrane contact.Cellular and molecular life sciences : CMLS · 2026Article
- Calcium-Dependent Protein Kinases in Plants: Structure, Signaling, and Multifaceted Regulatory Roles in Development and Stress Adaptation.International journal of molecular sciences · 2026Review
- Genomic survey and expression analysis of the CDPK genes in Liriodendron Chinense to explore their potential functions under multiple abiotic stresses.BMC genomics · 2026Article
- CaNature communications · 2025Article
- Interdependence of plasma membrane nanoscale dynamics of a kinase and its cognate substrate underlieseLife · 2025Article
- Arabidopsis Calcium Dependent Protein Kinase 3, and Its Orthologues OsCPK1, OsCPK15, and AcCPK16, Are Involved in Biotic and Abiotic Stresses.Plants (Basel, Switzerland) · 2025Article
- Article
- Genomic survey and evolution analysis of calcium-dependent protein kinases in plants and their stress-responsive patterns in populus.BMC genomics · 2024Article
- Genome-Wide Identification of the CDPK Gene Family and Their Involvement in Taproot Cracking in Radish.International journal of molecular sciences · 2023Article
- The putative myristoylome of Physcomitrium patens reveals conserved features of myristoylation in basal land plants.Plant cell reports · 2023Article
- The Roles of CDPKs as a Convergence Point of Different Signaling Pathways in Maize Adaptation to Abiotic Stress.International journal of molecular sciences · 2023Review
- Identification of theFrontiers in plant science · 2023Article
- Identification of Arabidopsis Protein Kinases That Harbor Functional Type 1 Peroxisomal Targeting Signals.Frontiers in cell and developmental biology · 2022Article
- The role of CDPKs in plant development, nutrient and stress signaling.Frontiers in genetics · 2022Review
- Calcium in peroxisomes: An essential messenger in an essential cell organelle.Frontiers in cell and developmental biology · 2022Review
- Molecular cloning and expression of AmCDPK from mangrove Avicennia marina under elevated temperature.Ecotoxicology (London, England) · 2020Article
- PROTEIN PHOSPHATASE 2A-B'Plant physiology · 2020Article
- Genome-wide investigation of calcium-dependent protein kinase gene family in pineapple: evolution and expression profiles during development and stress.BMC genomics · 2020Article
46 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Calcium-dependent protein kinases (CDPKs) are specific to plants and some protists. Their activation by calcium makes them important switches for the transduction of intracellular calcium signals. Here, we identify the subcellular targeting potentials for nine CDPK isoforms from Arabidopsis, as determined by expression of green fluorescent protein (GFP) fusions in transgenic plants. Subcellular locations were determined by fluorescence microscopy in cells near the root tip. Isoforms AtCPK3-GFP and AtCPK4-GFP showed a nuclear and cytosolic distribution similar to that of free GFP. Membrane fractionation experiments confirmed that these isoforms were primarily soluble. A membrane association was observed for AtCPKs 1, 7, 8, 9, 16, 21, and 28, based on imaging and membrane fractionation experiments. This correlates with the presence of potential N-terminal acylation sites, consistent with acylation as an important factor in membrane association. All but one of the membrane-associated isoforms targeted exclusively to the plasma membrane. The exception was AtCPK1-GFP, which targeted to peroxisomes, as determined by covisualization with a peroxisome marker. Peroxisome targeting of AtCPK1-GFP was disrupted by a deletion of two potential N-terminal acylation sites. The observation of a peroxisome-located CDPK suggests a mechanism for calcium regulation of peroxisomal functions involved in oxidative stress and lipid metabolism.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.