ArticleThe EMBO journal2003
A 10 residue motif at the C-terminus of the RNA pol II CTD is required for transcription, splicing and 3' end processing.
Article in The EMBO journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Identification and Functional Analysis of ThreeInsects · 2024Article
- N-terminal tagging of RNA Polymerase II shapes transcriptomes more than C-terminal alterations.iScience · 2024Article
- Epigenetic regulation of alternative splicing.American journal of cancer research · 2018Review
- Structural basis to stabilize the domain motion of BARD1-ARD BRCT by CstF50.Scientific reports · 2017Article
- Localization of RNAPII and 3' end formation factor CstF subunits on C. elegans genes and operons.Transcription · 2016Article
- Function and control of RNA polymerase II C-terminal domain phosphorylation in vertebrate transcription and RNA processing.Molecular and cellular biology · 2014Article
- Kin28 regulates the transient association of Mediator with core promoters.Nature structural & molecular biology · 2014Article
- Alternative splicing in plants--coming of age.Trends in plant science · 2012Review
- The RNA polymerase II CTD coordinates transcription and RNA processing.Genes & development · 2012Review
- Prolonged α-amanitin treatment of cells for studying mutated polymerases causes degradation of DSIF160 and other proteins.RNA (New York, N.Y.) · 2012Article
- Hexameric architecture of CstF supported by CstF-50 homodimerization domain structure.RNA (New York, N.Y.) · 2011Article
- Chromatin density and splicing destiny: on the cross-talk between chromatin structure and splicing.The EMBO journal · 2010Article
- Rates of in situ transcription and splicing in large human genes.Nature structural & molecular biology · 2009Article
- The Iws1:Spt6:CTD complex controls cotranscriptional mRNA biosynthesis and HYPB/Setd2-mediated histone H3K36 methylation.Genes & development · 2008Article
- Targeting tat inhibitors in the assembly of human immunodeficiency virus type 1 transcription complexes.Journal of virology · 2008Article
- The splicing factor SC35 has an active role in transcriptional elongation.Nature structural & molecular biology · 2008Article
- Serine-7 of the RNA polymerase II CTD is specifically required for snRNA gene expression.Science (New York, N.Y.) · 2007Article
- Splicing- and cleavage-independent requirement of RNA polymerase II CTD for mRNA release from the transcription site.The Journal of cell biology · 2007Article
- The C-terminal domain of RNA Pol II helps ensure that editing precedes splicing of the GluR-B transcript.RNA (New York, N.Y.) · 2007Article
- The conserved AAUAAA hexamer of the poly(A) signal can act alone to trigger a stable decrease in RNA polymerase II transcription velocity.RNA (New York, N.Y.) · 2006Article
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Authors and funding
4 authors.
Funding
Abstract
The RNA polymerase II C-terminal heptad repeat domain (CTD) is essential for normal transcription and co-transcriptional processing of mRNA precursors. The mammalian CTD comprises 52 heptads whose consensus, YSPTSPS, is conserved throughout eukaryotes, followed by a 10 amino acid C-terminal sequence that is conserved only among vertebrates. Here we show that surprisingly, the heptad repeats are not sufficient to support efficient transcription, pre-mRNA processing or full cell viability. In addition to the heptads, the 10 amino acid C-terminal motif is essential for high level transcription, splicing and poly(A) site cleavage. Efficient mRNA synthesis from a transiently transfected reporter gene required the C-terminal motif plus between 16 and 25 heptad repeats from either the N- or C-terminal half of the CTD. Twenty-seven consensus heptads plus the C-terminal motif also supported efficient mRNA synthesis but not cell viability.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.