ArticleEukaryotic cell2003
CF45-1, a secreted protein which participates in Dictyostelium group size regulation.
Article in Eukaryotic cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 35 citations in OpenAlex.
- Transcriptomics informed discovery of developmentally essential transcription factors.Biology open · 2025Article
- Cell dispersal by localized degradation of a chemoattractant.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- DPF is a cell-density sensing factor, with cell-autonomous and non-autonomous functions during Dictyostelium growth and development.BMC biology · 2019Article
- A telomerase with novel non-canonical roles: TERT controls cellular aggregation and tissue size in Dictyostelium.PLoS genetics · 2019Article
- Extracellular signaling in Dictyostelium.The International journal of developmental biology · 2019Review
- The Use of Diffusion Calculations and Monte Carlo Simulations to Understand the Behavior of Cells inComputational and structural biotechnology journal · 2019Review
- Controlling organism size by regulating constituent cell numbers.Proceedings of the ... IEEE Conference on Decision & Control. IEEE Conference on Decision & Control · 2018Article
- Loss of FrmB results in increased size of developmental structures during the multicellular development of Dictyostelium cells.Journal of microbiology (Seoul, Korea) · 2017Article
- Different CHD chromatin remodelers are required for expression of distinct gene sets and specific stages during development of Dictyostelium discoideum.Development (Cambridge, England) · 2013Article
- Regulation of aggregate size and pattern by adenosine and caffeine in cellular slime molds.BMC developmental biology · 2012Article
- Autonomous and non-autonomous traits mediate social cooperation in Dictyostelium discoideum.Journal of biosciences · 2011Article
- Cell density sensing and size determination.Development, growth & differentiation · 2011Review
- A cell number counting factor alters cell metabolism.Communicative & integrative biology · 2009Article
- Dictyostelium cells bind a secreted autocrine factor that represses cell proliferation.BMC biochemistry · 2009Article
- A protein with similarity to PTEN regulates aggregation territory size by decreasing cyclic AMP pulse size during Dictyostelium discoideum development.Eukaryotic cell · 2008Article
- Combining experiments and modelling to understand size regulation in Dictyostelium discoideum.Journal of the Royal Society, Interface · 2008Review
- Article
- The secreted Dictyostelium protein CfaD is a chalone.Journal of cell science · 2008Article
- CnrN regulates Dictyostelium group size using a counting factor-independent mechanism.Communicative & integrative biology · 2008Article
- A cell number-counting factor regulates levels of a novel protein, SslA, as part of a group size regulation mechanism in Dictyostelium.Eukaryotic cell · 2007Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Developing Dictyostelium cells aggregate to form fruiting bodies containing typically 2 x 10(4) cells. To prevent the formation of an excessively large fruiting body, streams of aggregating cells break up into groups if there are too many cells. The breakup is regulated by a secreted complex of polypeptides called counting factor (CF). Countin and CF50 are two of the components of CF. Disrupting the expression of either of these proteins results in cells secreting very little detectable CF activity, and as a result, aggregation streams remain intact and form large fruiting bodies, which invariably collapse. We find that disrupting the gene encoding a third protein present in crude CF, CF45-1, also results in the formation of large groups when cells are grown with bacteria on agar plates and then starve. However, unlike countin(-) and cf50(-) cells, cf45-1(-) cells sometimes form smaller groups than wild-type cells when the cells are starved on filter pads. The predicted amino acid sequence of CF45-1 has some similarity to that of lysozyme, but recombinant CF45-1 has no detectable lysozyme activity. In the exudates from starved cells, CF45-1 is present in a approximately 450-kDa fraction that also contains countin and CF50, suggesting that it is part of a complex. Recombinant CF45-1 decreases group size in colonies of cf45-1(-) cells with a 50% effective concentration (EC(50)) of approximately 8 ng/ml and in colonies of wild-type and cf50(-) cells with an EC(50) of approximately 40 ng/ml. Like countin(-) and cf50(-) cells, cf45-1(-) cells have high levels of cytosolic glucose, high cell-cell adhesion, and low cell motility. Together, the data suggest that CF45-1 participates in group size regulation in Dictyostelium.
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