ArticleGene therapy2003
Focused ultrasound (HIFU) induces localized enhancement of reporter gene expression in rabbit carotid artery.
Article in Gene therapy, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 80 citations in OpenAlex.
- Immunomodulation and targeted drug delivery with high intensity focused ultrasound (HIFU): Principles and mechanisms.Pharmacology & therapeutics · 2023Review
- Synergies between therapeutic ultrasound, gene therapy and immunotherapy in cancer treatment.Advanced drug delivery reviews · 2021Review
- Article
- Investigation of temporal vascular effects induced by focused ultrasound treatment with speckle-variance optical coherence tomography.Biomedical optics express · 2014Article
- Inhibitory effects of subcutaneous tumors in nude mice mediated by low-frequency ultrasound and microbubbles.Oncology letters · 2014Article
- Insight concerning the mechanism of therapeutic ultrasound facilitating gene delivery: increasing cell membrane permeability or interfering with intracellular pathways?Human gene therapy · 2014Article
- MR-guided focused ultrasound surgery, present and future.Medical physics · 2013Review
- Efficient microbubble- and ultrasound-mediated plasmid DNA delivery into a specific rat liver lobe via a targeted injection and acoustic exposure using a novel ultrasound system.Molecular pharmaceutics · 2012Article
- Explorations of high-intensity therapeutic ultrasound and microbubble-mediated gene delivery in mouse liver.Gene therapy · 2011Article
- Physical methods of nucleic acid transfer: general concepts and applications.British journal of pharmacology · 2009Review
- Sonoporation of the minicircle-VEGF(165) for wound healing of diabetic mice.Pharmaceutical research · 2009Article
- Ultrasonic-activated micellar drug delivery for cancer treatment.Journal of pharmaceutical sciences · 2009Review
- Ultrasound mediated delivery of drugs and genes to solid tumors.Advanced drug delivery reviews · 2008Review
- Key factors that affect sonoporation efficiency in in vitro settings: the importance of standing wave in sonoporation.Biochemical and biophysical research communications · 2007Article
- Ultrasonically targeted delivery into endothelial and smooth muscle cells in ex vivo arteries.Journal of controlled release : official journal of the Controlled Release Society · 2007Article
- Virus-mediated gene transfer to induce therapeutic angiogenesis: where do we stand?International journal of nanomedicine · 2007Review
- Using magnetic forces to enhance non-viral gene transfer to airway epithelium in vivo.Gene therapy · 2006Article
- The role of cavitation in acoustically activated drug delivery.Journal of controlled release : official journal of the Controlled Release Society · 2005Article
- Nonviral gene transfer to skeletal, smooth, and cardiac muscle in living animals.American journal of physiology. Cell physiology · 2005Review
- Intracellular delivery of Bak BH3 peptide by microbubble-enhanced ultrasound.Pharmaceutical research · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
Abstract
The development of accurate, safe, and efficient gene delivery remains a major challenge towards the realization of gene therapeutic prevention and treatment of cardiovascular diseases. In this study, we investigated the ability of high-intensity focused ultrasound (HIFU), a form of mechanical wave transmission, to act as a noninvasive tool for the enhancement of in vivo gene transfer into rabbit carotid arteries. Segments of the common carotid arteries of New Zealand white rabbits were isolated and infused with plasmid DNA encoding the reporter beta-galactosidase either with or without the addition of ultrasound contrast agent consisting of small (approximately 2-5 microm) gas-filled human albumin microspheres to augment cavitation. Infused arteries were exposed to pulsed ultrasound for 1 min (frequency 0.85 MHz, burst length 50 ms, repetition frequency 1 Hz, duration 60 s, peak pressure amplitude of 15 MPa). At 6.3 MPa, HIFU enhanced gene expression eight-fold, and 17.5-fold in the presence of contrast. We found increasing amounts of beta-galactosidase expression in the carotid vessel with increasing pressure amplitude. This dose-response relation was present with and without contrast. Without contrast, no vessel damage was detected up to 15 MPa, while the addition of contrast induced side effects above a threshold of 6.3 MPa peak pressure. The entire procedure was feasible and safe for the animals, and the results suggest that HIFU has the potential to assist in the noninvasive spatial regulation of gene transfer into the vascular system.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.