ArticleNucleic acids research2003
Gene expression analysis using single molecule detection.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Single-pair fluorescence resonance energy transfer analysis of mRNA transcripts for highly sensitive gene expression profiling in near real time.Analytical chemistry · 2013Article
- Article
- PCR-free detection of genetically modified organisms using magnetic capture technology and fluorescence cross-correlation spectroscopy.PloS one · 2009Article
- Quantitative screening of single copies of human papilloma viral DNA without amplification.Analytical chemistry · 2006Article
- Limitations of mRNA amplification from small-size cell samples.BMC genomics · 2005Article
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Authors and funding
20 authors.
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Abstract
Recent developments of single molecule detection techniques and in particular the introduction of fluorescence correlation spectroscopy (FCS) led to a number of important applications in biological research. We present a unique approach for the gene expression analysis using dual-color cross-correlation. The expression assay is based on gene-specific hybridization of two dye-labeled DNA probes to a selected target gene. The counting of the dual-labeled molecules within the solution allows the quantification of the expressed gene copies in absolute numbers. As detection and analysis by FCS can be performed at the level of single molecules, there is no need for any type of amplification. We describe the gene expression assay and present data demonstrating the capacity of this novel technology. In order to prove the gene specificity, we performed experiments with gene-depleted total cDNA. The biological application was demonstrated by quantifying selected high, medium and low abundant genes in cDNA prepared from HL-60 cells.
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