ArticleNucleic acids research2003
Dendrimeric coating of glass slides for sensitive DNA microarrays analysis.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 189 citations in OpenAlex.
- Review
- Biocompatibility of Phosphorus Dendrimers and Their Antibacterial Properties as Potential Agents for Supporting Wound Healing.Molecular pharmaceutics · 2025Article
- Editorial for the Glassy Materials and Micro/Nano Devices Section.Micromachines · 2025Article
- Interplay between Nanoparticles and Phosphorus Dendrimers, and Their Properties.Molecules (Basel, Switzerland) · 2023Review
- Phosphorus-carrying cascade molecules: inner architecture to biomedical applications.Turkish journal of chemistry · 2023Article
- The DendrisCHIPDiagnostics (Basel, Switzerland) · 2022Article
- Which Current and Novel Diagnostic Avenues for Bacterial Respiratory Diseases?Frontiers in microbiology · 2020Review
- Innovative DendrisChipsDiagnostics (Basel, Switzerland) · 2018Article
- Digital DNA detection based on a compact optofluidic laser with ultra-low sample consumption.Lab on a chip · 2016Article
- Automated and Multiplexed Soft Lithography for the Production of Low-Density DNA Microarrays.Microarrays (Basel, Switzerland) · 2016Article
- Immobilization techniques for microarray: challenges and applications.Sensors (Basel, Switzerland) · 2014Review
- Shrink-induced silica multiscale structures for enhanced fluorescence from DNA microarrays.Langmuir : the ACS journal of surfaces and colloids · 2014Article
- Ultraviolet light enhances the bovine serum albumin fixation for acid fast bacilli stain.PloS one · 2014Article
- Drop drying on surfaces determines chemical reactivity - the specific case of immobilization of oligonucleotides on microarrays.BMC biophysics · 2013Article
- A comparative transcriptomic, fluxomic and metabolomic analysis of the response of Saccharomyces cerevisiae to increases in NADPH oxidation.BMC genomics · 2012Article
- Direct immobilization of DNA probes on non-modified plastics by UV irradiation and integration in microfluidic devices for rapid bioassay.Analytical and bioanalytical chemistry · 2012Article
- Facile DNA immobilization on surfaces through a catecholamine polymer.Angewandte Chemie (International ed. in English) · 2011Article
- Gold nanoparticle-assisted single base-pair mismatch discrimination on a microfluidic microarray device.Biomicrofluidics · 2010Article
- Multifunctional Dendrimer-templated Antibody Presentation on Biosensor Surfaces for Improved Biomarker Detection.Advanced functional materials · 2010Article
- Multiplexed bead-based mesofluidic system for detection of food-borne pathogenic bacteria.Applied and environmental microbiology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Successful use and reliability of microarray technology is highly dependent on several factors, including surface chemistry parameters and accessibility of cDNA targets to the DNA probes fixed onto the surface. Here, we show that functionalisation of glass slides with homemade dendrimers allow production of more sensitive and reliable DNA microarrays. The dendrimers are nanometric structures of size-controlled diameter with aldehyde function at their periphery. Covalent attachment of these spherical reactive chemical structures on amino-silanised glass slides generates a reactive approximately 100 A layer onto which amino-modified DNA probes are covalently bound. This new grafting chemistry leads to the formation of uniform and homogenous spots. More over, probe concentration before spotting could be reduced from 0.2 to 0.02 mg/ml with PCR products and from 20 to 5 micro M with 70mer oligonucleotides without affecting signal intensities after hybridisation with Cy3- and Cy5-labelled targets. More interestingly, while the binding capacity of captured probes on dendrimer-activated glass surface (named dendrislides) is roughly similar to other functionalised glass slides from commercial sources, detection sensitivity was 2-fold higher than with other available DNA microarrays. This detection limit was estimated to 0.1 pM of cDNA targets. Altogether, these features make dendrimer-activated slides ideal for manufacturing cost-effective DNA arrays applicable for gene expression and detection of mutations.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.