ArticleNucleic acids research2003
Impact of surface chemistry and blocking strategies on DNA microarrays.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 157 citations in OpenAlex.
- Blood Biomarker Detection Using Integrated Microfluidics with Optical Label-Free Biosensor.Sensors (Basel, Switzerland) · 2024Article
- Blocking Nonspecific Interactions Using Y-Shape Poly(ethylene glycol).International journal of molecular sciences · 2023Article
- Innovative DendrisChipsDiagnostics (Basel, Switzerland) · 2018Article
- Achieving reproducible performance of electrochemical, folding aptamer-based sensors on microelectrodes: challenges and prospects.Analytical chemistry · 2014Article
- Enhancing the detection limit of nanoscale biosensors via topographically selective functionalization.Analytical chemistry · 2014Article
- Optimized light-directed synthesis of aptamer microarrays.Analytical chemistry · 2013Article
- Drop drying on surfaces determines chemical reactivity - the specific case of immobilization of oligonucleotides on microarrays.BMC biophysics · 2013Article
- Label-free electrical detection of pyrophosphate generated from DNA polymerase reactions on field-effect devices.The Analyst · 2012Article
- Facile DNA immobilization on surfaces through a catecholamine polymer.Angewandte Chemie (International ed. in English) · 2011Article
- Strand-specific transcriptome profiling with directly labeled RNA on genomic tiling microarrays.BMC molecular biology · 2011Article
- Diagnostic devices as biomaterials: a review of nucleic acid and protein microarray surface performance issues.Journal of biomaterials science. Polymer edition · 2008Review
- Label-free detection of biomolecules on microarrays using surface-colloid interaction.Analytical biochemistry · 2007Article
- An Activated GOPS-poly-Engineering in life sciences · 2005Article
- Rapid and simultaneous detection of human hepatitis B virus and hepatitis C virus antibodies based on a protein chip assay using nano-gold immunological amplification and silver staining method.BMC infectious diseases · 2005Article
- Optimization of gold nanoparticle-based DNA detection for microarrays.Journal of fluorescence · 2005Article
- Microarrays and breast cancer clinical studies: forgetting what we have not yet learnt.Breast cancer research : BCR · 2005Review
- Microarray segmentation methods significantly influence data precision.Nucleic acids research · 2004Article
- Real-time detection of polymerase activity using supercritical angle fluorescence.Journal of fluorescence · 2004Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The surfaces and immobilization chemistries of DNA microarrays are the foundation for high quality gene expression data. Four surface modification chemistries, poly-L-lysine (PLL), 3-glycidoxypropyltrimethoxysilane (GPS), DAB-AM-poly(propyleminime hexadecaamine) dendrimer (DAB) and 3-aminopropyltrimethoxysilane (APS), were evaluated using cDNA and oligonucleotide sub-arrays. Two un-silanized glass surfaces, RCA-cleaned and immersed in Tris-EDTA buffer were also studied. DNA on amine-modified surfaces was fixed by UV (90 mJ/cm(2)), while DNA on GPS-modified surfaces was immobilized by covalent coupling. Arrays were blocked with either succinic anhydride (SA), bovine serum albumin (BSA) or left unblocked prior to hybridization with labeled PCR product. Quality factors evaluated were surface affinity for cDNA versus oligonucleotides, spot and background intensity, spotting concentration and blocking chemistry. Contact angle measurements and atomic force microscopy were preformed to characterize surface wettability and morphology. The GPS surface exhibited the lowest background intensity regardless of blocking method. Blocking the arrays did not affect raw spot intensity, but affected background intensity on amine surfaces, BSA blocking being the lowest. Oligonucleotides and cDNA on unblocked GPS-modified slides gave the best signal (spot-to-background intensity ratio). Under the conditions evaluated, the unblocked GPS surface along with amine covalent coupling was the most appropriate for both cDNA and oligonucleotide microarrays.
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