ArticleNucleic acids research2003
Methylation of CpG dinucleotides in the open reading frame of a testicular germ cell-specific intronless gene, Tact1/Actl7b, represses its expression in somatic cells.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 54 citations in OpenAlex.
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- Selection on synonymous sites: the unwanted transcript hypothesis.Nature reviews. Genetics · 2024Review
- The Regulatory Role of Rac1, a Small Molecular Weight GTPase, in the Development of Diabetic Retinopathy.Journal of clinical medicine · 2019Review
- Epigenetic signature and enhancer activity of the human APOE gene.Human molecular genetics · 2013Article
- Distinct DNA methylation dynamics of spermatogenic cell-specific intronless genes is associated with CpG content.PloS one · 2012Article
- Epigenetic modification of TLRs in leukocytes is associated with increased susceptibility to Salmonella enteritidis in chickens.PloS one · 2012Article
- Demethylation of the coding region triggers the activation of the human testis-specific PDHA2 gene in somatic tissues.PloS one · 2012Article
- The impact of intragenic CpG content on gene expression.Nucleic acids research · 2010Article
- Developmental methylation program and concerted expression of Stx11 in mouse tissues.Mammalian genome : official journal of the International Mammalian Genome Society · 2009Article
- Comparative anti-androgenic actions of vinclozolin and flutamide on transgenerational adult onset disease and spermatogenesis.Reproductive toxicology (Elmsford, N.Y.) · 2008Article
- Gene silencing by RNAi in mouse Sertoli cells.Reproductive biology and endocrinology : RB&E · 2008Article
- Epigenetic programming of the germ line: effects of endocrine disruptors on the development of transgenerational disease.Reproductive biomedicine online · 2008Review
- Article
- An unmethylated 3' promoter-proximal region is required for efficient transcription initiation.PLoS genetics · 2007Article
- cAMP-responsive element in TATA-less core promoter is essential for haploid-specific gene expression in mouse testis.Nucleic acids research · 2005Article
- Isolation and characterization of an Arabidopsis mutant that overaccumulates O-Acetyl-L-Ser.Plant physiology · 2004Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Methylation of CpG islands spanning promoter regions is associated with control of gene expression. However, it is considered that methylation of exonic CpG islands without promoter is not related to gene expression, because such exonic CpG islands are usually distant from the promoter. Whether methylation of exonic CpG islands near the promoter, as in the case of a CpG-rich intronless gene, causes repression of the promoter remains unknown. To gain insight into this issue, we investigated the distribution and methylation status of CpG dinucleotides in the mouse Tact1/Actl7b gene, which is intronless and expressed exclusively in testicular germ cells. The region upstream to the gene was poor in CpG, with CpG dinucleotides absent from the core promoter. However, a CpG island was found inside the open reading frame (ORF). Analysis of the methylation status of the Tact1/Actl7b gene including the 5'-flanking area demonstrated that all CpG sites were methylated in somatic cells, whereas these sites were unmethylated in the Tact1/Actl7b-positive testis. Trans fection experiments with in vitro-methylated constructs indicated that methylation of the ORF but not 5' upstream repressed Tact1/Actl7b promoter activity in somatic cells. Similar effects of ORF methylation on the promoter activity were observed in testicular germ cells. These are the first results indicating that methylation of the CpG island in the ORF represses its promoter in somatic cells and demethylation is necessary for gene expression in spermatogenic cells.
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