ArticlePflugers Archiv : European journal of physiology2003
Blood sampling methodology is crucial for precise measurement of plasma catecholamines concentrations in mice.
Article in Pflugers Archiv : European journal of physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 75 citations in OpenAlex.
- Acute Comparative Analysis of Anesthetic Methods in Neonatal Mice (Mus musculus) Using Ultrasonic Vocalizations and Biochemical Markers.Journal of the American Association for Laboratory Animal Science : JAALAS · 2026Article
- Article
- Blood Biomarkers in Takotsubo Syndrome Point to an Emerging Role for Inflammaging in Endothelial Pathophysiology.Biomolecules · 2023Article
- CNP Promotes Antiarrhythmic Effects via Phosphodiesterase 2.Circulation research · 2023Article
- Article
- Clinical, Physiologic, and Behavioral Evaluation of Permanently Catheterized NMRI Mice.Journal of the American Association for Laboratory Animal Science : JAALAS · 2019Article
- Vascular smooth muscle cell contraction and relaxation in the isolated aorta: a critical regulator of large artery compliance.Physiological reports · 2019Article
- Blood collection in unstressed, conscious, and freely moving mice through implantation of catheters in the jugular vein: a new simplified protocol.Physiological reports · 2018Article
- Control of (pre)-analytical aspects in immunoassay measurements of metabolic hormones in rodents.Endocrine connections · 2018Review
- Euthanasia of laboratory mice: Are isoflurane and sevoflurane real alternatives to carbon dioxide?PloS one · 2018Article
- Stroke-induced chronic systolic dysfunction driven by sympathetic overactivity.Annals of neurology · 2017Article
- Article
- Type, duration, and incidence of pathologic findings after retroorbital bleeding of mice by experienced and novice personnel.Journal of the American Association for Laboratory Animal Science : JAALAS · 2015Article
- Assessment of MRI Contrast Agent Kinetics via Retro-Orbital Injection in Mice: Comparison with Tail Vein Injection.PloS one · 2015Article
- Manual versus automated blood sampling: impact of repeated blood sampling on stress parameters and behavior in male NMRI mice.Laboratory animals · 2014Article
- Impact of heparin and short term anesthesia on the quantification of cytokines in laboratory mouse plasma.Acta veterinaria Scandinavica · 2014Article
- Physiological and pathological impact of blood sampling by retro-bulbar sinus puncture and facial vein phlebotomy in laboratory mice.PloS one · 2014Article
- Decrease in heart adrenoceptor gene expression and receptor number as compensatory tool for preserved heart function and biological rhythm in M(2) KO animals.Naunyn-Schmiedeberg's archives of pharmacology · 2012Article
- Regulation of β-adrenergic control of heart rate by GTP-cyclohydrolase 1 (GCH1) and tetrahydrobiopterin.Cardiovascular research · 2012Article
- Enhanced catecholamine release in mice expressing PKB/SGK-resistant GSK3.Pflugers Archiv : European journal of physiology · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Epinephrine (E) and norepinephrine (NE) play a major role in regulating metabolism and cardiovascular physiology. Both are secreted in response to stress and their measurement in plasma allows the study of sympathoadrenal function. Several studies investigating sympathoadrenal physiology are conducted using mice. Review of the literature revealed that basal mouse NE and E plasma concentrations range within 4-140 nM depending on the blood sampling method. Such variability doesn't allow study comparison and may conceal catecholamine variations in response to stress. Therefore, our aim was to determine a reliable sampling method to measure mouse plasma catecholamine concentrations. Results showed that arterial catheterization is the most accurate sampling method: E and NE basal levels were similar to those found in humans (1.1+/-0.3 nM and 4.1+/-0.5 nM, respectively). Retro-orbital bleeding led to analogous results. On the contrary, decapitation was stressful for mice and consequently NE and E concentrations were high (24.6+/-2.7 nM and 27.3+/-3.8 nM, respectively). These different bleeding methods were compared in terms of their ability to detect sympathoadrenal system stimulation (cold-pressure test). With catheter and retro-orbital samplings the expected increase in NE and E levels was easily perceived. In contrast, with decapitation no significant change in E was detected. In conclusion, arterial-catheter and retro-orbital blood sampling methods appear to be the most accurate procedures for studying the sympathetic nervous system in mice in both unstressed and stressed conditions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.