ArticlePlanta2003
Subcellular localization of two types of ferrochelatase in cucumber.
Article in Planta, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 70 citations in OpenAlex.
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- Localization of heme biosynthesis in the diatomFrontiers in plant science · 2025Article
- Chloroplast protein StFC-II was manipulated by aHorticulture research · 2024Article
- Improved plant biomass production under low nitrogen conditions through conditional accumulation of the second messenger, guanosine tetraphosphate, in chloroplasts and mitochondria.Frontiers in plant science · 2024Article
- Retrograde signaling in plants: A critical review focusing on the GUN pathway and beyond.Plant communications · 2023Review
- Advances in Iron Retrograde Signaling Mechanisms and Uptake Regulation in Photosynthetic Organisms.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Glutamate 1-semialdehyde aminotransferase is connected to GluTR by GluTR-binding protein and contributes to the rate-limiting step of 5-aminolevulinic acid synthesis.The Plant cell · 2022Article
- Ferroportin 3 is a dual-targeted mitochondrial/chloroplast iron exporter necessary for iron homeostasis in Arabidopsis.The Plant journal : for cell and molecular biology · 2021Article
- The Role of Tetrapyrrole- and GUN1-Dependent Signaling on Chloroplast Biogenesis.Plants (Basel, Switzerland) · 2021Review
- Anatomic features, tolerance index, secondary metabolites and protein content of chickpea (Physiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Proteomic analysis of haem-binding protein fromPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
- Overexpression of chloroplast-targeted ferrochelatase 1 results in aPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
- Dual or Not Dual?-Comparative Analysis of Fluorescence Microscopy-Based Approaches to Study Organelle Targeting Specificity of Nuclear-Encoded Plant Proteins.Frontiers in plant science · 2018Article
- Plant Frataxin in Metal Metabolism.Frontiers in plant science · 2018Article
- Evolution of the Tetrapyrrole Biosynthetic Pathway in Secondary Algae: Conservation, Redundancy and Replacement.PloS one · 2016Article
- Allocation of Heme Is Differentially Regulated by Ferrochelatase Isoforms in Arabidopsis Cells.Frontiers in plant science · 2016Article
- Organization of chlorophyll biosynthesis and insertion of chlorophyll into the chlorophyll-binding proteins in chloroplasts.Photosynthesis research · 2015Review
- Tetrapyrrole-based drought stress signalling.Plant biotechnology journal · 2015Review
- Increased expression of Fe-chelatase leads to increased metabolic flux into heme and confers protection against photodynamically induced oxidative stress.Plant molecular biology · 2014Article
- Mitochondrial iron transport and homeostasis in plants.Frontiers in plant science · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is widely believed that ferrochelatase (protoheme ferrolyase, EC 4.99.1.1), which catalyzes the insertion of ferrous ion into protoporphyrin IX to form protoheme, exists in both plastids and mitochondria of higher plants. By in vitro import assay with isolated pea (Pisum sativum L.) organelles, it has been proposed that one of two isoforms of ferrochelatase (type 1) is dual-targeted into both plastids and mitochondria, and functions for heme biosynthesis in the both organelles. Recently, however, mitochondrial targeting of ferrochelatase is being disputed since pea mitochondria appeared to accept a variety of chloroplast proteins including the type-1 ferrochelatase of Arabidopsis thaliana (L.) Heynh. To clarify the precise subcellular localization of ferrochelatase in higher plants, here we investigated the subcellular localization of two types of ferrochelatase (CsFeC1 and CsFeC2) in cucumber (Cucumis sativus L.). In cotyledons, a significant level of specific ferrochelatase activity was detected in thylakoid membranes, but only a trace level of activity was detectable in mitochondria. Western blot analysis with specific antibodies showed that anti-CsFeC2 antiserum cross-reacted with plastids in photosynthetic and non-photosynthetic tissues. Anti-CsFeC1 did not cross-react with mitochondria, but CsFeC1 was clearly detectable in plastids from non-photosynthetic tissues. In situ transient-expression assays using green fluorescent protein demonstrated that, as well as CsFeC2, the N-terminal transit peptide of CsFeC1 targeted the fusion protein solely into plastids, but not into mitochondria. These results demonstrated that in cucumber both CsFeC1 and CsFeC2 are solely targeted into plastids, but not into mitochondria. Screening of a cucumber genomic or cDNA library did not allow any other ferrochelatase homologous gene to be isolated. The data presented here imply the reconsideration of mitochondrial heme biosynthesis in higher plants.
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