ArticleProceedings of the National Academy of Sciences of the United States of America2003
A large-scale, gene-driven mutagenesis approach for the functional analysis of the mouse genome.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
59 citing papers in PubMed, 144 citations in OpenAlex.
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- Cytochrome P450 family proteins as potential biomarkers for ovarian granulosa cell damage in mice with premature ovarian failure.International journal of clinical and experimental pathology · 2018Article
- Inactivation of the miR-183/96/182 Cluster Decreases the Severity of Pseudomonas aeruginosa-Induced Keratitis.Investigative ophthalmology & visual science · 2016Article
- Spry2 regulates signalling dynamics and terminal bud branching behaviour during lung development.Genetics research · 2015Article
- Modeling the study of DNA damage responses in mice.Methods in molecular biology (Clifton, N.J.) · 2015Review
- Plant homeodomain finger protein 2 promotes bone formation by demethylating and activating Runx2 for osteoblast differentiation.Cell research · 2014Article
- Intersectin-1s: an important regulator of cellular and molecular pathways in lung injury.Pulmonary circulation · 2013Article
- Gene-trap mutagenesis using Mol/MSM-1 embryonic stem cells from MSM/Ms mice.Mammalian genome : official journal of the International Mammalian Genome Society · 2013Article
- Inactivation of the microRNA-183/96/182 cluster results in syndromic retinal degeneration.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Article
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Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A major challenge of the postgenomic era is the functional characterization of every single gene within the mammalian genome. In an effort to address this challenge, we assembled a collection of mutations in mouse embryonic stem (ES) cells, which is the largest publicly accessible collection of such mutations to date. Using four different gene-trap vectors, we generated 5,142 sequences adjacent to the gene-trap integration sites (gene-trap sequence tags; http://genetrap.de) from >11,000 ES cell clones. Although most of the gene-trap vector insertions occurred randomly throughout the genome, we found both vector-independent and vector-specific integration "hot spots." Because >50% of the hot spots were vector-specific, we conclude that the most effective way to saturate the mouse genome with gene-trap insertions is by using a combination of gene-trap vectors. When a random sample of gene-trap integrations was passaged to the germ line, 59% (17 of 29) produced an observable phenotype in transgenic mice, a frequency similar to that achieved by conventional gene targeting. Thus, gene trapping allows a large-scale and cost-effective production of ES cell clones with mutations distributed throughout the genome, a resource likely to accelerate genome annotation and the in vivo modeling of human disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.