ArticleJournal of clinical microbiology2003
Automated extraction and quantification of human cytomegalovirus DNA in whole blood by real-time PCR assay.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 92 citations in OpenAlex.
- Molecular characterization of cytomegalovirus based on glycoprotein B and N among solid organ and hematopoietic stem cell transplant recipients in Jordan.Iranian journal of microbiology · 2026Article
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- High tacrolimus intra-patient variability is associated with graft rejection, andWorld journal of gastroenterology · 2018Article
- Correlation between systemic lupus erythematosus and cytomegalovirus infection detected by different methods.Clinical rheumatology · 2015Article
- An Association between BK Virus Replication in Bone Marrow and Cytopenia in Kidney-Transplant Recipients.Journal of transplantation · 2014Article
- Prospective evaluation of a new automated nucleic acid extraction system using routine clinical respiratory specimens.Journal of medical virology · 2012Article
- Expression of O⁶-methylguanine-DNA methyltransferase in childhood medulloblastoma.Journal of neuro-oncology · 2011Article
- Rapid quantitation of cytomegalovirus DNA in whole blood by a new molecular assay based on automated sample preparation and real-time PCR.Medical microbiology and immunology · 2010Article
- Monitoring human cytomegalovirus infection with nested PCR: comparison of positive rates in plasma and leukocytes and with quantitative PCR.Virology journal · 2010Article
- Comparative evaluation of a commercially available automated system for extraction of viral DNA from whole blood: application to monitoring of epstein-barr virus and cytomegalovirus load.Journal of clinical microbiology · 2009Article
- Comparison of quantitative cytomegalovirus real-time PCR in whole blood and pp65 antigenemia assay: clinical utility of CMV real-time PCR in hematopoietic stem cell transplant recipients.Journal of Korean medical science · 2009Article
- Monitoring of cytomegalovirus reactivation in bone marrow transplant recipients by real-time PCR.Pathology oncology research : POR · 2008Article
- Detection of cytomegalovirus (CMV) DNA in EDTA whole-blood samples: evaluation of the quantitative artus CMV LightCycler PCR kit in conjunction with automated sample preparation.Journal of clinical microbiology · 2008Article
- Prediction of cytomegalovirus (CMV) plasma load from evaluation of CMV whole-blood load in samples from renal transplant recipients.Journal of clinical microbiology · 2008Article
- Simultaneous quantification of Epstein-Barr virus, cytomegalovirus, and human herpesvirus 6 DNA in samples from transplant recipients by multiplex real-time PCR assay.Journal of clinical microbiology · 2007Article
- Cytomegalovirus DNA in semen of men seeking fertility evaluation in Abidjan, Côte d'Ivoire.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2007Article
- Real-time PCR in clinical microbiology: applications for routine laboratory testing.Clinical microbiology reviews · 2006Review
- Current and developing technologies for monitoring agents of bioterrorism and biowarfare.Clinical microbiology reviews · 2005Review
- Comparison of five methods for extraction of Legionella pneumophila from respiratory specimens.Journal of clinical microbiology · 2004Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The measurement of human cytomegalovirus (HCMV) DNA in blood is becoming the standard method for monitoring HCMV infection in immune-suppressed and unsuppressed patients. As various blood compartments can be used, we have compared the HCMV DNA measured in whole blood (WB), peripheral blood leukocytes (PBL), and plasma by real-time PCR. We tested 286 samples: HCMV DNA was extracted automatically from WB and PBL with the MagNA Pure instrument (Roche Molecular Biochemicals) and manually from plasma samples. The HCMV DNA from WB, PBL, and plasma was measured by real-time Light Cycler PCR. Primers and probe were located in the UL 83 region. HCMV DNA was detected more frequently in WB (88.5%) than in the PBL (65.7%) (P < 0.0001) or the plasma (55.2%) (P < 0.0001). There was a good correlation between the positive results in WB and in PBL (r = 0.68; P < 0.0001), and 3.15 log(10) genome copies in 200000 PBL, equivalent to the threshold value of 50 pp65-positive polymorphonuclear cells per 200000 leukocytes, was equivalent to 3.4 log(10) genome copies in 200 microl of WB. WB was shown to be suitable for automated extraction and the quantitation of HCMV DNA by real-time Light Cycler PCR by analysis of serial samples from representative patients of various populations. This system may be very useful for monitoring of immune-suppressed and unsuppressed patients.
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