ArticleJournal of clinical microbiology2003
Real-time PCR assay to detect smallpox virus.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 101 citations in OpenAlex.
- Decoding the mechanophysiology for inhaled onset of smallpox with model-based implications for mpox spread.Scientific reports · 2025Article
- Development of Smallpox Antibody Testing and Surveillance Following Smallpox Vaccination in the Republic of Korea.Vaccines · 2024Article
- The establishment of a recombinase polymerase amplification technique for the detection of mouse poxvirus.BMC veterinary research · 2023Article
- Review
- The electrochemical detection of bioterrorism agents: a review of the detection, diagnostics, and implementation of sensors in biosafety programs for Class A bioweapons.Microsystems & nanoengineering · 2021Review
- Microfluidic devices for detection of RNA viruses.Reviews in medical virology · 2021Review
- Ultraspecific analyte detection by direct kinetic fingerprinting of single molecules.Trends in analytical chemistry : TRAC · 2020Article
- [Journal of virology · 2017Article
- Article
- Exposure of rhesus monkeys to cowpox virus Brighton Red by large-particle aerosol droplets results in an upper respiratory tract disease.The Journal of general virology · 2016Article
- Small particle aerosol inoculation of cowpox Brighton Red in rhesus monkeys results in a severe respiratory disease.Virology · 2015Article
- Molecular Detection of 10 of the Most Unwanted Alien Forest Pathogens in Canada Using Real-Time PCR.PloS one · 2015Article
- Article
- Optimization of peptide-based ELISA for serological diagnostics: a retrospective study of human monkeypox infection.Vector borne and zoonotic diseases (Larchmont, N.Y.) · 2012Article
- Evaluation of monkeypox disease progression by molecular imaging.The Journal of infectious diseases · 2011Article
- Article
- Species-specific identification of variola, monkeypox, cowpox, and vaccinia viruses by multiplex real-time PCR assay.Journal of virological methods · 2011Article
- Comparative analysis of monkeypox virus infection of cynomolgus macaques by the intravenous or intrabronchial inoculation route.Journal of virology · 2011Article
- Article
- Development of real-time PCR assay for specific detection of cowpox virus.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2010Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed a highly sensitive and specific assay for the rapid detection of smallpox virus DNA on both the Smart Cycler and LightCycler platforms. The assay is based on TaqMan chemistry with the orthopoxvirus hemagglutinin gene used as the target sequence. With genomic DNA purified from variola virus Bangladesh 1975, the limit of detection was estimated to be approximately 25 copies on both machines. The assay was evaluated in a blinded study with 322 coded samples that included genomic DNA from 48 different isolates of variola virus; 25 different strains and isolates of camelpox, cowpox, ectromelia, gerbilpox, herpes, monkeypox, myxoma, rabbitpox, raccoonpox, skunkpox, vaccinia, and varicella-zoster viruses; and two rickettsial species at concentrations mostly ranging from 100 fg/ microl to 1 ng/ microl. Contained within those 322 samples were variola virus DNA, obtained from purified viral preparations, at concentrations of 1 fg/ microl to 1 ng/ microl. On the Smart Cycler platform, 2 samples with false-positive results were detected among the 116 samples not containing variola virus tested; i.e., the overall specificity of the assay was 98.3%. On the LightCycler platform, five samples with false-positive results were detected (overall specificity, 95.7%). Of the 206 samples that contained variola virus DNA ranging in concentrations from 100 fg/ microl to 1 ng/ microl, 8 samples were considered negative on the Smart Cycler platform and 1 sample was considered negative on the LightCycler platform. Thus, the clinical sensitivities were 96.1% for the Smart Cycler instrument and 99.5% for the LightCycler instrument. The vast majority of these samples were derived from virus-infected cell cultures and variola virus-infected tissues; thus, the DNA material contained both viral DNA and cellular DNA. Of the 43 samples that contained purified variola virus DNA ranging in concentration from 1 fg/ microl to 1 ng/ microl, the assay correctly detected the virus in all 43 samples on both the Smart Cycler and the LightCycler platforms. The assay may be useful for the early detection of smallpox virus infections should such infections occur as a result of a deliberate or an accidental recurrence.
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