ArticleJournal of clinical microbiology2003
Genomic approach to identification of Mycobacterium bovis diagnostic antigens in cattle.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 55 citations in OpenAlex.
- Serological and epidemiological investigation of Mycobacterium avium subspecies paratuberculosis in bovines in Pakistan.Animal bioscience · 2024Article
- Screening of recombinant proteins as antigens in indirect ELISA for diagnosis of bovine tuberculosis.SpringerPlus · 2012Article
- Serum antibody responses to 10 Mycobacterium tuberculosis proteins, purified protein derivative, and old tuberculin in natural and experimental tuberculosis in rhesus monkeys.Clinical and vaccine immunology : CVI · 2011Article
- Comparative genomics of Esx genes from clinical isolates of Mycobacterium tuberculosis provides evidence for gene conversion and epitope variation.Infection and immunity · 2011Article
- Screening of predicted secreted antigens from Mycobacterium bovis identifies potential novel differential diagnostic reagents.Clinical and vaccine immunology : CVI · 2010Article
- Screening of predicted secreted antigens from Mycobacterium bovis reveals the immunodominance of the ESAT-6 protein family.Infection and immunity · 2010Article
- Assessment of Mycobacterium tuberculosis OmpATb as a novel antigen for the diagnosis of bovine tuberculosis.Clinical and vaccine immunology : CVI · 2009Article
- Identification of novel Mycobacterium bovis antigens by dissection of crude protein fractions.Clinical and vaccine immunology : CVI · 2009Article
- Whole genome identification of Mycobacterium tuberculosis vaccine candidates by comprehensive data mining and bioinformatic analyses.BMC medical genomics · 2008Article
- Optimizing antigen cocktails for detection of Mycobacterium bovis in herds with different prevalences of bovine tuberculosis: ESAT6-CFP10 mixture shows optimal sensitivity and specificity.Journal of clinical microbiology · 2006Article
- Assessing the serodiagnostic potential of 35 Mycobacterium tuberculosis proteins and identification of four novel serological antigens.Journal of clinical microbiology · 2005Article
- Specific T-cell epitopes for immunoassay-based diagnosis of Mycobacterium tuberculosis infection.Journal of clinical microbiology · 2004Article
- Protein-protein interactions of proteins from the ESAT-6 family of Mycobacterium tuberculosis.Journal of bacteriology · 2004Article
- Cell envelope protein PPE68 contributes to Mycobacterium tuberculosis RD1 immunogenicity independently of a 10-kilodalton culture filtrate protein and ESAT-6.Infection and immunity · 2004Article
- Gamma interferon-based immunodiagnosis of tuberculosis: comparison between whole-blood and enzyme-linked immunospot methods.Journal of clinical microbiology · 2004Article
- Human T-cell responses to the RD1-encoded protein TB27.4 (Rv3878) from Mycobacterium tuberculosis.Immunology · 2003Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Differential delayed-type hypersensitivity skin testing with tuberculin purified protein derivatives from Mycobacterium bovis and M. avium is the standard for diagnosing bovine tuberculosis. However, improved tests based on defined, specific antigens are urgently needed. In the present study, a combination of bioinformatics, molecular biology, and bovine models of infection were used to screen mycobacterial proteins for their potential as diagnostic reagents which could be used in a whole-blood assay for diagnosis of tuberculosis. Initial screening of 28 proteins selected in silico and expressed as recombinants in Escherichia coli indicated that CFP-10, ESAT-6, TB27.4, TB16.2, TB15.8, and TB10.4 induced strong gamma interferon responses in experimentally infected cattle. A more thorough investigation over time in two groups of animals infected with a high (10(6) CFU) and a low (10(4) CFU) dose of M. bovis revealed that, for both groups, the strength of the in vitro response to individual antigens varied greatly over time. However, combining the results for ESAT-6, CFP-10, and TB27.4, possibly supplemented with TB10.4, gave sensitivities at different infection stages close to those obtained with M. bovis purified protein derivative. Importantly, while responsiveness to ESAT-6 and CFP-10 correlated strongly for individual samples, the same was not the case for ESAT-6 and TB27.4 responsiveness. The results suggest that combinations of specific antigens such as these have great potential in development of optimized diagnostic systems for bovine tuberculosis.
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