ArticleJournal of clinical microbiology2003
Mycobacterial interspersed repetitive unit typing of Mycobacterium tuberculosis compared to IS6110-based restriction fragment length polymorphism analysis for investigation of apparently clustered cases of tuberculosis.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Utilizing rapid multiple-locus variable-number tandem-repeat analysis typing to aid control of hospital-acquired Clostridium difficile Infection: a multicenter study.Journal of clinical microbiology · 2012Trial
- Using Whole-genome Sequencing to Determine the Timing of Secondary Tuberculosis in British Columbia, Canada.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2021Article
- Laboratory Methods in Molecular Epidemiology: Bacterial Infections.Microbiology spectrum · 2018Review
- Molecular Epidemiology of Tuberculosis in British Columbia, Canada: A 10-Year Retrospective Study.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2018Article
- Diversity ofBioMed research international · 2018Article
- Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria.Clinical microbiology reviews · 2016Review
- Current methods in the molecular typing of Mycobacterium tuberculosis and other mycobacteria.BioMed research international · 2014Review
- Whole-genome sequencing to delineate Mycobacterium tuberculosis outbreaks: a retrospective observational study.The Lancet. Infectious diseases · 2013Article
- Molecular epidemiology of Mycobacterium tuberculosis complex in Singapore, 2006-2012.PloS one · 2013Article
- Article
- Genomics and outbreak investigation: from sequence to consequence.Genome medicine · 2013Review
- Characterization of microevolution events in Mycobacterium tuberculosis strains involved in recent transmission clusters.Journal of clinical microbiology · 2011Article
- Investigating transmission of Mycobacterium bovis in the United Kingdom in 2005 to 2008.Journal of clinical microbiology · 2011Article
- Article
- Synchronous Comparison of Mycobacterium tuberculosis Epidemiology Strains by "MIRU-VNTR" and "MIRU-VNTR and Spoligotyping" Technique.Avicenna journal of medical biotechnology · 2010Article
- Prospective universal application of mycobacterial interspersed repetitive-unit-variable-number tandem-repeat genotyping to characterize Mycobacterium tuberculosis isolates for fast identification of clustered and orphan cases.Journal of clinical microbiology · 2009Article
- Factors associated with differences between conventional contact tracing and molecular epidemiology in study of tuberculosis transmission and analysis in the city of Barcelona, Spain.Journal of clinical microbiology · 2009Article
- Evaluation of the new advanced 15-loci MIRU-VNTR genotyping tool in Mycobacterium tuberculosis molecular epidemiology studies.BMC microbiology · 2008Article
- Optimized molecular resolution of cross-contamination alerts in clinical mycobacteriology laboratories.BMC microbiology · 2008Article
- Assessment of an optimized mycobacterial interspersed repetitive- unit-variable-number tandem-repeat typing system combined with spoligotyping for population-based molecular epidemiology studies of tuberculosis.Journal of clinical microbiology · 2007Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An evaluation of the utility of IS6110-based restriction fragment length polymorphism (RFLP) typing compared to a combination of variable number tandem repeat (VNTR) typing and mycobacterial interspersed repetitive unit (MIRU) typing was undertaken. A total of 53 patient isolates of Mycobacterium tuberculosis from four presumed episodes of cross-infection were examined. Genomic DNA was extracted from the isolates by a cetyl trimethylammonium bromide method. The number of copies of tandem repeats of the five loci ETR(A) to ETR(E) and 12 MIRU loci was determined by PCR amplification and agarose gel electrophoresis of the amplicons. VNTR typing identified the major clusters of strains in the three investigations in which they occurred (each representing a different evolutionary clade: 32333, 42235, and 32433). The majority of unrelated isolates (by epidemiology and RFLP typing) were also identified by VNTR typing. The concordance between the RFLP and MIRU typing was complete, with the exception of two isolates with RFLP patterns that differed by one band each from the rest of the major epidemiologically linked groups of isolates in investigation A. All of these isolates had identical MIRU and VNTR types. A further pair of isolates differed in the number of tandem repeat copies at two MIRU alleles but had identical RFLP patterns. The speed of the combined VNTR and MIRU typing approach enabled results for some of the investigations to be supplied in "real time," influencing choices in contact tracing. The ease of comparison of results of MIRU and VNTR typing, which are recorded as single multidigit numbers, was also found to greatly facilitate investigation management and the communication of results to health care professionals.
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