ArticleThe Journal of biological chemistry2003
Plasmid accumulation reduces life span in Saccharomyces cerevisiae.
Article in The Journal of biological chemistry, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 66 citations in OpenAlex.
- DNA Circles as Vehicles for Genes to Evade Chromosomal Discipline.Genome biology and evolution · 2025Article
- Noncanonical and reversible cysteine ubiquitination prevents the overubiquitination of PEX5 at the peroxisomal membrane.PLoS biology · 2024Article
- Peroxisomal NAD(H) Homeostasis in the YeastBiomolecules · 2023Article
- Senescence in yeast is associated with amplified linear fragments of chromosome XII rather than ribosomal DNA circle accumulation.PLoS biology · 2023Article
- Spindle Position Checkpoint Kinase Kin4 Regulates Organelle Transport inBiomolecules · 2023Article
- The dynamin-related protein Vps1 and the peroxisomal membrane protein Pex27 function together during peroxisome fission.Journal of cell science · 2023Article
- Article
- DNA circles promote yeast ageing in part through stimulating the reorganization of nuclear pore complexes.eLife · 2022Article
- Understanding the Impact of Industrial Stress Conditions on Replicative Aging inFrontiers in fungal biology · 2021Review
- Genomic Instability and Cellular Senescence: Lessons From the Budding Yeast.Frontiers in cell and developmental biology · 2020Review
- Advances in quantitative biology methods for studying replicative aging inTranslational medicine of aging · 2020Article
- Behead and live long or the tale of cathepsin L.Yeast (Chichester, England) · 2018Article
- Article
- Evidence for the hallmarks of human aging in replicatively aging yeast.Microbial cell (Graz, Austria) · 2016Review
- Article
- Asymmetrical Inheritance of Plasmids Depends on Dynamic Cellular Geometry and Volume Exclusion Effects.PloS one · 2015Article
- Role of asymmetric cell division in lifespan control in Saccharomyces cerevisiae.FEMS yeast research · 2014Review
- Article
- Yeast sirtuins and the regulation of aging.FEMS yeast research · 2014Review
- Dietary restriction and mitochondrial function link replicative and chronological aging in Saccharomyces cerevisiae.Experimental gerontology · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Aging in the yeast Saccharomyces cerevisiae is under the control of multiple pathways. The production and accumulation of extrachromosomal rDNA circles (ERCs) is one pathway that has been proposed to bring about aging in yeast. To test this proposal, we have developed a plasmid-based model system to study the role of DNA episomes in reduction of yeast life span. Recombinant plasmids containing different replication origins, cis-acting partitioning elements, and selectable marker genes were constructed and analyzed for their effects on yeast replicative life span. Plasmids containing the ARS1 replication origin reduce life span to the greatest extent of the plasmids analyzed. This reduction in life span is partially suppressed by a CEN4 centromeric element on ARS1 plasmids. Plasmids containing a replication origin from the endogenous yeast 2 mu circle also reduce life span, but to a lesser extent than ARS1 plasmids. Consistent with this, ARS1 and 2 mu origin plasmids accumulate in approximately 7-generation-old cells, but ARS1/CEN4 plasmids do not. Importantly, ARS1 plasmids accumulate to higher levels in old cells than 2 mu origin plasmids, suggesting a correlation between plasmid accumulation and life span reduction. Reduction in life span is neither an indirect effect of increased ERC levels nor the result of stochastic cessation of growth. The presence of a fully functional 9.1-kb rDNA repeat on plasmids is not required for, and does not augment, reduction in life span. These findings support the view that accumulation of DNA episomes, including episomes such as ERCs, cause cell senescence in yeast.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.