ArticleSexually transmitted infections2003
Expression of the cytolethal distending toxin in a geographically diverse collection of Haemophilus ducreyi clinical isolates.
Article in Sexually transmitted infections, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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3 authors at 2 institutions in 2 countries.
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Abstract
objectiveTo screen a collection of isolates of Haemophilus ducreyi for expression of the cytolethal distending toxin (CDT).
methods45 clinical isolates of H ducreyi were screened for cytotoxic activity by examining the effect of culture supernatants on Hela cells. Expression was confirmed using immunoblotting with CDT specific monoclonal antibodies and the presence of the cdt genes determined by amplification of the cdt genes in a multiplex polymerase chain assay.
resultsOf the 45 clinical isolates, six isolates from differing geographical origins did not demonstrate cytotoxic activity. Expression of CDT was also not detected in these six isolates using immunoblotting and the genes cdtA, cdtB, and cdtC were not amplified using PCR. The remaining isolates demonstrated cytotoxic activity, expressed the CDT proteins, and the presence of the cdt genes was confirmed.
conclusionsCDT is considered a virulence factor of H ducreyi but was found to be absent in 13% of isolates from different geographical origins.
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