ArticleApplied and environmental microbiology2003
Detection of bacteria carrying the stx2 gene by in situ loop-mediated isothermal amplification.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
42 citing papers in PubMed, 1 synthesis or guideline pooled it, 143 citations in OpenAlex.
- Prevalence of bovine paratuberculosis in Chinese cattle populations: a meta-analysis.Frontiers in cellular and infection microbiology · 2024Pooled it
- Development of ELISA Using Phage-Displayed Stx2 Mini-Body for Detection of STEC Antigen in Field Farming Pig Samples.Microorganisms · 2025Article
- Recent Advancements in Technologies to Detect EnterohaemorrhagicJournal of microbiology and biotechnology · 2023Review
- An Overview of the Public Health Challenges in Diagnosing and Controlling Human Foodborne Pathogens.Vaccines · 2023Review
- Molecular Methods for Identification and Quantification of Foodborne Pathogens.Molecules (Basel, Switzerland) · 2022Review
- One-Day Molecular Detection ofFoods (Basel, Switzerland) · 2021Article
- Comparison of loop-mediated isothermal amplification and conventional PCR tests for diagnosis of common Brucella species.BMC research notes · 2020Article
- Isothermal Nucleic Acid Amplification Techniques and Their Use in Bioanalysis.Biochemistry. Biokhimiia · 2020Review
- Colorimetric biosensors for point-of-care virus detections.Materials science for energy technologies · 2020Review
- Article
- In situ loop-mediated isothermal amplification (LAMP) for identification of Plasmodium species in wide-range thin blood smears.Malaria journal · 2018Article
- Smartphone-Based in-Gel Loop-Mediated Isothermal Amplification (gLAMP) System Enables Rapid Coliphage MS2 Quantification in Environmental Waters.Environmental science & technology · 2018Article
- Visual Detection of Enterohemorrhagic Escherichia coli O157:H7 Using Loop-Mediated Isothermal Amplification.Electronic physician · 2016Article
- Emerging Loop-Mediated Isothermal Amplification-Based Microchip and Microdevice Technologies for Nucleic Acid Detection.ACS biomaterials science & engineering · 2016Article
- Article
- Development of Primer Sets for Loop-Mediated Isothermal Amplification that Enables Rapid and Specific Detection of Streptococcus dysgalactiae, Streptococcus uberis and Streptococcus agalactiae.International journal of environmental research and public health · 2015Article
- Evaluation of a loop-mediated isothermal amplification suite for the rapid, reliable, and robust detection of Shiga toxin-producing Escherichia coli in produce.Applied and environmental microbiology · 2014Article
- Development and Assessment of Loop-Mediated Isothermal Amplification (LAMP) Assay for the Diagnosis of Human Visceral Leishmaniasis in Iran.Iranian journal of parasitology · 2014Article
- Establishment and application of a loop-mediated isothermal amplification method for simple, specific, sensitive and rapid detection of Toxoplasma gondii.The Journal of veterinary medical science · 2014Article
- Development of a multiplex loop-mediated isothermal amplification assay to detect shiga toxin-producing Escherichia coli in cattle.Journal of veterinary science · 2014Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new in situ DNA amplification technique for microscopic detection of bacteria carrying a specific gene is described. Loop-mediated isothermal amplification (LAMP) was used to detect stxA(2) in Escherichia coli O157:H7 cells. The mild permeabilization conditions and low isothermal temperature used in the in situ LAMP method caused less cell damage than in situ PCR. It allowed use of fluorescent antibody labeling in the bacterial mixture after the DNA amplification for identification of E. coli O157:H7 cells with an stxA(2) gene. Higher-contrast images were obtained with this method than with in situ PCR.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.