ArticleApplied and environmental microbiology2003
PCR assay of the groEL gene for detection and differentiation of Bacillus cereus group cells.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 68 citations in OpenAlex.
- Multidrug-resistant Bacillus cereus in retail poultry meat: molecular insights into toxigenicity and antimicrobial resistance.BMC microbiology · 2026Article
- Molecular detection of foodborne pathogens in Ardabil's milk supply.BMC veterinary research · 2025Article
- Meat and meat products as potential sources of emerging MDR Bacillus cereus: groEL gene sequencing, toxigenic and antimicrobial resistance.BMC microbiology · 2024Article
- Detection and Characterisation of EndosymbiontTropical life sciences research · 2023Article
- Development of peptide nucleic acid-based bead array technology for Bacillus cereus detection.Scientific reports · 2023Article
- Phenotypic diagnosis and genotypic identification ofVeterinary world · 2023Article
- Identification of Genetic Markers for the Detection ofFoods (Basel, Switzerland) · 2022Article
- Profiling of Bacillus cereus on Canadian grain.PloS one · 2021Article
- Multi-locus sequence typing and virulence profile inItalian journal of food safety · 2020Article
- Detection and Enumeration of Spore-Forming Bacteria in Powdered Dairy Products.Frontiers in microbiology · 2017Review
- Association of Genotyping of Bacillus cereus with Clinical Features of Post-Traumatic Endophthalmitis.PloS one · 2016Article
- Assessment of genetic diversity of Bacillus spp. isolated from eutrophic fish culture pond.3 Biotech · 2015Article
- Differentiation of Bacillus anthracis, B. cereus, and B. thuringiensis on the basis of the csaB gene reflects host source.Applied and environmental microbiology · 2013Article
- Identification of new single nucleotide polymorphism-based markers for inter- and intraspecies discrimination of obligate bacterial parasites (Pasteuria spp.) of invertebrates.Applied and environmental microbiology · 2011Article
- The analysis of groEL gene in Salmonella enterica subspecies enterica serovar Typhimurium isolated from avians by PCR-Restriction Fragment Length Polymorphism method.Veterinary research communications · 2011Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Strains of species in the Bacillus cereus group are potentially enterotoxic. Thus, the detection of all B. cereus group strains is important. As 16S ribosomal DNA sequence analysis cannot adequately differentiate species of the B. cereus group, we explored the potential of the groEL gene as a phylogenetic marker. A phylogenetic analysis of the groEL sequences of 78 B. cereus group strains revealed that the B. cereus group strains were split into two major clusters, one including six B. mycoides and one B. pseudomycoides (cluster II) and the other including two B. mycoides and the rest of the B. cereus group strains (cluster I). Cluster I was further differentiated into two subclusters, Ia and Ib. The sodA gene sequences of representative strains from different clusters were also compared. The phylogenetic tree constructed from the sodA sequences showed substantial similarity to the tree constructed from the groEL sequences. Based on the groEL sequences, a PCR assay for detection and identification of B. cereus group strains was developed. Subsequent restriction fragment length polymorphism (RFLP) analysis verified the PCR amplicons and the differentiation of the B. cereus group strains. RFLP with MboI was identical for all the B. cereus group strains analyzed, while RFLP with MfeI or PstI classified all B. cereus and B. thuringiensis strains into two groups. All cluster II B. mycoides and B. pseudomycoides strains could be discriminated from other B. cereus group bacteria by restriction analysis with TspRI.
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