ArticleGene therapy2003
Transgene delivery of plasmid DNA to smooth muscle cells and macrophages from a biostable polymer-coated stent.
Article in Gene therapy, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 52 citations in OpenAlex.
- Backbone-Degradable Polymers Prepared by Chemical Vapor Deposition.Angewandte Chemie (International ed. in English) · 2017Article
- Modulation of NO and ROS production by AdiNOS transduced vascular cells through supplementation with L-Arg and BH4: implications for gene therapy of restenosis.Atherosclerosis · 2013Article
- Adenoviral vector tethering to metal surfaces via hydrolyzable cross-linkers for the modulation of vector release and transduction.Biomaterials · 2013Article
- Polyelectrolyte multilayers promote stent-mediated delivery of DNA to vascular tissue.Biomacromolecules · 2013Article
- Gene delivery technologies for cardiac applications.Gene therapy · 2012Review
- Layered PLG scaffolds for in vivo plasmid delivery.Biomaterials · 2009Article
- Multilayered polyelectrolyte assemblies as platforms for the delivery of DNA and other nucleic acid-based therapeutics.Advanced drug delivery reviews · 2008Review
- Local delivery of gene vectors from bare-metal stents by use of a biodegradable synthetic complex inhibits in-stent restenosis in rat carotid arteries.Circulation · 2008Article
- Delivery of large biopharmaceuticals from cardiovascular stents: a review.Biomacromolecules · 2007Review
- Cardiovascular gene therapy: current status and therapeutic potential.British journal of pharmacology · 2007Review
- Release of plasmid DNA from intravascular stents coated with ultrathin multilayered polyelectrolyte films.Biomacromolecules · 2006Article
- Cardiovascular gene delivery: The good road is awaiting.Advanced drug delivery reviews · 2006Review
- Bisphosphonate-mediated gene vector delivery from the metal surfaces of stents.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Nonviral gene transfer to skeletal, smooth, and cardiac muscle in living animals.American journal of physiology. Cell physiology · 2005Review
- Endovascular Gene Delivery from a Stent Platform: Gene- Eluting Stents.Angiology: open accessArticle
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Metallic stents coated with a polyurethane emulsion containing plasmid DNA were implanted in rabbit iliac arteries to evaluate transgene delivery and expression in the vessel wall. The expression of the plasmid-encoded marker genes, beta-galactosidase, luciferase and green fluorescence protein (GFP), were evaluated at 7 days after implantation. In all cases, plasmid transfer was confined to the vessel wall at the site of stent implantation, plasmid DNA was not observed in vessel segments immediately proximal or distal to the stent and dissemination of plasmid DNA to lung, liver or spleen was not observed. Expression of transgenes occurred only in vessel segments in contact with the stent and analysis of the GFP expression pattern revealed a high frequency of marker protein-positive cells occurring at or near the luminal surface. The extent of transgene expression was dependent upon the quantity of DNA loaded onto the stent and no signal was detected in vessel segments that received polymer-coated stents lacking plasmid DNA. Of significance, colocalization studies identified transgene expression not only in vascular smooth muscle cells but also in macrophages. Hence, polymer-coated stents provide a new capability for transgene delivery to immune cells that are believed to contribute to the development of in-stent restenosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.