ArticleGene therapy2003
Effective transduction and stable transgene expression in human blood cells by a third-generation lentiviral vector.
Article in Gene therapy, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed.
- Development of KoRV-pseudotyped lentiviral vectors for efficient gene transfer into freshly isolated immune cells.Gene therapy · 2024Article
- CD155 and CD112 as possible therapeutic targets ofOncology letters · 2022Article
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- Efficient Non-viral Gene Delivery into Human Hematopoietic Stem Cells by Minicircle Sleeping Beauty Transposon Vectors.Molecular therapy : the journal of the American Society of Gene Therapy · 2018Article
- Efficacy and Safety of Doubly-Regulated Vaccinia Virus in a Mouse Xenograft Model of Multiple Myeloma.Molecular therapy oncolytics · 2017Article
- Salicylate, diflunisal and their metabolites inhibit CBP/p300 and exhibit anticancer activity.eLife · 2016Article
- A novel long non-coding RNA in the rheumatoid arthritis risk locus TRAF1-C5 influences C5 mRNA levels.Genes and immunity · 2016Article
- HTLV-1 induces a Th1-like state in CD4+CCR4+ T cells.The Journal of clinical investigation · 2014Article
- Establishment of single-cell screening system for the rapid identification of transcriptional modulators involved in direct cell reprogramming.Nucleic acids research · 2012Article
- Article
- Article
- Vinblastine induces acute, cell cycle phase-independent apoptosis in some leukemias and lymphomas and can induce acute apoptosis in others when Mcl-1 is suppressed.Molecular cancer therapeutics · 2010Article
- Gene set enrichment analysis provides insight into novel signalling pathways in breast cancer stem cells.British journal of cancer · 2010Article
- Article
- Construction of a high-performance human fetal liver-derived lentiviral cDNA library.Molecular and cellular biochemistry · 2008Article
- Article
- Article
- Nuclear import of the preintegration complex is blocked upon infection by human immunodeficiency virus type 1 in mouse cells.Journal of virology · 2007Article
- Retrovirus-mediated tk gene therapy of implanted human breast cancer in nude mice under the regulation of Tet-On.Cancer gene therapy · 2006Article
- HTLV-I basic leucine zipper factor gene mRNA supports proliferation of adult T cell leukemia cells.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Difficulty in gene transduction of human blood cells, including hematopoietic stem cells, has hampered the development of gene therapy applications for hematological disorders, encouraging the development and use of new gene delivery systems. In this study, we used a third-generation self-inactivating (SIN) lentiviral vector system based on human immunodeficiency virus type 1 (HIV-1) to improve transduction efficiency and prevent vector-related toxicity. The transduction efficiency of the HIV-1-based vector was compared directly with the Moloney murine leukemia virus (MLV) SIN vector in human leukemia cell lines. Initial transduction efficiencies were almost 100% for the HIV and less than 50% for the MLV vectors. Similar results were observed in 11 types of primary cells obtained from leukemia or myeloma patients. Transgene expression persisted for 8 weeks in cells transduced with the HIV vector, but declined with the MLV vector. In addition, resting peripheral blood lymphocytes and CD34(+) hematopoietic cells were transduced successfully with the HIV vector, but not with the MLV vector. Finally, we confirmed vector gene integration in almost all colony-forming cells transduced with the HIV vector, but not with the MLV vector. In conclusion, this lentiviral vector is an excellent gene transduction system for human blood cells because of its high gene transduction and host chromosome integration efficiency.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.