ArticleFunctional & integrative genomics2003
Global nutritional profiling for mutant and chemical mode-of-action analysis in filamentous fungi.
Article in Functional & integrative genomics, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 52 citations in OpenAlex.
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- Development of an auto-inducible expression system by nitrogen sources switching based on the nitrogen catabolite repression regulation.Microbial cell factories · 2022Article
- Biocontrol ofInsects · 2021Article
- Characterization of the promoter of the nitrate transporter-encoding gene nrtA in Aspergillus nidulans.Molecular genetics and genomics : MGG · 2020Article
- Bioremediation of Dichlorodiphenyltrichloroethane (DDT)-Contaminated Agricultural Soils: Potential of Two Autochthonous Saprotrophic Fungal Strains.Applied and environmental microbiology · 2019Article
- Co-inoculum of Beauveria brongniartii and B. bassiana shows in vitro different metabolic behaviour in comparison to single inoculums.Scientific reports · 2017Article
- An Endohyphal Bacterium (Frontiers in microbiology · 2017Article
- Phenotype MicroArrays as a complementary tool to next generation sequencing for characterization of tree endophytes.Frontiers in microbiology · 2015Article
- Phenotypic profiling of Scedosporium aurantiacum, an opportunistic pathogen colonizing human lungs.PloS one · 2015Article
- Cells in cells: morphogenetic and metabolic strategies conditioning rice infection by the blast fungus Magnaporthe oryzae.Protoplasma · 2014Review
- Principles of carbon catabolite repression in the rice blast fungus: Tps1, Nmr1-3, and a MATE-family pump regulate glucose metabolism during infection.PLoS genetics · 2012Article
- Comparative genomic analysis of human fungal pathogens causing paracoccidioidomycosis.PLoS genetics · 2011Article
- Review: Global nutrient profiling by Phenotype MicroArrays: a tool complementing genomic and proteomic studies in conidial fungi.Journal of Zhejiang University. Science. B · 2010Review
- GLN3 encodes a global regulator of nitrogen metabolism and virulence of C. albicans.Fungal genetics and biology : FG & B · 2008Article
- Global carbon utilization profiles of wild-type, mutant, and transformant strains of Hypocrea jecorina.Applied and environmental microbiology · 2006Article
- Harnessing natural diversity to probe metabolic pathways.PLoS genetics · 2005Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe a method for gene function discovery and chemical mode-of-action analysis via nutrient utilization using a high throughput Nutritional Profiling platform suitable for filamentous microorganisms. We have optimized the growth conditions for each fungal species to produce reproducible optical density growth measurements in microtiter plates. We validated the Nutritional Profiling platform using a nitrogen source utilization assay to analyze 21 Aspergillus nidulans strains with mutations in the master nitrogen regulatory gene, areA. Analysis of these data accurately reproduced expected results and provided new data to demonstrate that this platform is suitable for fine level phenotyping of filamentous fungi. Next, we analyzed the differential responses of two fungal species to a glutamine synthetase inhibitor, illustrating chemical mode-of-action analysis. Finally, a comparative phenotypic study was performed to characterize carbon catabolite repression in four fungal species using a carbon source utilization assay. The results demonstrate differentiation between two Aspergillus species and two diverse plant pathogens and provide a wealth of new data on fungal nutrient utilization. Thus, these assays can be used for gene function and chemical mode-of-action analysis at the whole organism level as well as interspecies comparisons in a variety of filamentous fungi. Additionally, because uniform distribution of growth within wells is maintained, comparisons between yeast and filamentous forms of a single organism can be performed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.