ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2003
BIBAC and TAC clones containing potato genomic DNA fragments larger than 100 kb are not stable in Agrobacterium.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- AnMicroorganisms · 2026Article
- Transposon-derived genome editors in plants: from compact nucleases to large-fragment integration and regeneration strategies.Engineering in life sciences · 2026Review
- Through the lens of bioenergy crops: advances, bottlenecks, and promises of plant engineering.The Plant journal : for cell and molecular biology · 2025Review
- Plant engineering: advances, bottlenecks, and promise.The Plant journal : for cell and molecular biology · 2025Article
- Split selectable marker systems utilizing inteins facilitate gene stacking in plants.Communications biology · 2023Article
- Drought-responsive WRKY transcription factor genesFrontiers in plant science · 2022Article
- Molecular and FISH analyses of a 53-kbp intact DNA fragment inserted by biolistics in wheat (Triticum aestivum L.) genome.Plant cell reports · 2017Article
- Article
- Transformation of rice with large maize genomic DNA fragments containing high content repetitive sequences.Plant cell reports · 2015Article
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- Genomic resources for gene discovery, functional genome annotation, and evolutionary studies of maize and its close relatives.Genetics · 2013Article
- Article
- Bacterial artificial chromosome libraries of pulse crops: characteristics and applications.Journal of biomedicine & biotechnology · 2012Review
- A pair of new BAC and BIBAC vectors that facilitate BAC/BIBAC library construction and intact large genomic DNA insert exchange.Plant methods · 2011Article
- A large insert Thellungiella halophila BIBAC library for genomics and identification of stress tolerance genes.Plant molecular biology · 2010Article
- Transformation of rice with long DNA-segments consisting of random genomic DNA or centromere-specific DNA.Transgenic research · 2007Article
- Construction of BAC and BIBAC libraries from sunflower and identification of linkage group-specific clones by overgo hybridization.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2006Article
- Three minimum tile paths from bacterial artificial chromosome libraries of the soybean (Glycine max cv. 'Forrest'): tools for structural and functional genomics.Plant methods · 2006Article
- Rearrangements of large-insert T-DNAs in transgenic rice.Molecular genetics and genomics : MGG · 2005Article
- Construction and characterization of a plant transformation-competent BIBAC library of the black Sigatoka-resistant banana Musa acuminata cv. Tuu Gia (AA).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2005Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Development of efficient methods to transfer large DNA fragments into plants will greatly facilitate the map-based cloning of genes. The recently developed BIBAC and TAC vectors have shown potential to deliver large DNA fragments into plants via Agrobacterium-mediated transformation. Here we report that BIBAC and TAC clones containing potato genomic DNA fragments larger than 100 kb are not stable in Agrobacterium. We tested the possible factors that may cause instability, including the insert sizes of the BIBAC and TAC constructs, potato DNA fragments consisting of highly repetitive or largely single-copy DNA sequences, different Agrobacterium transformation methods and different Agrobacterium strains. The insert sizes of the potato BIBAC and TAC constructs were found to be critical to their stability in Agrobacterium. All constructs containing a potato DNA fragment larger than 100 kb were not stable in any of the four tested Agrobacterium strains, including two recA deficient strains. We developed a transposon-based technique that can be used to efficiently subclone a BAC insert into two to three BIBAC/TAC constructs to circumvent the instability problem.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.