ArticleJournal of bacteriology2003
Identification of a regulated alkaline phosphatase, a cell surface-associated lipoprotein, in Mycobacterium smegmatis.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 64 citations in OpenAlex.
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- Integrated analysis of transposon insertion sequencing and pangenome reveals core and lineage-specific essential genes inMicrobial genomics · 2026Article
- Functional genomics reveals strain-specific genetic requirements conferring hypoxic growth ineLife · 2025Article
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- Effective Small Molecule Antibacterials from a Novel Anti-Protein Secretion Screen.Microorganisms · 2021Article
- A mycobacterial DivIVA domain-containing protein involved in cell length and septation.Microbiology (Reading, England) · 2020Article
- Potential therapeutic approaches for a sleeping pathogen: tuberculosis a case for bioinorganic chemistry.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2020Review
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- Genome-wide identification of essential genes in Mycobacterium intracellulare by transposon sequencing - Implication for metabolic remodeling.Scientific reports · 2020Article
- Dual control of RegX3 transcriptional activity by SenX3 and PknB.The Journal of biological chemistry · 2019Article
- Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways.mSystems · 2019Article
- Identification and Expression Analysis of an Atypical Alkaline Phosphatase inFrontiers in microbiology · 2018Article
- Methionine AntagonizesFrontiers in cellular and infection microbiology · 2018Article
- Long-Chain Fatty Acyl Coenzyme A Ligase FadD2 Mediates Intrinsic Pyrazinamide Resistance in Mycobacterium tuberculosis.Antimicrobial agents and chemotherapy · 2017Article
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- Targeting intracellular p-aminobenzoic acid production potentiates the anti-tubercular action of antifolates.Scientific reports · 2016Article
- Gene deletions in Mycobacterium bovis BCG stimulate increased CD8+ T cell responses.Infection and immunity · 2014Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Although alkaline phosphatases are common in a wide variety of bacteria, there has been no prior evidence for alkaline phosphatases in Mycobacterium smegmatis. Here we report that transposon insertions in the pst operon, encoding homologues of an inorganic phosphate transporter, leads to constitutive expression of a protein with alkaline phosphatase activity. DNA sequence analysis revealed that M. smegmatis does indeed have a phoA gene that shows high homology to other phoA genes. The M. smegmatis phoA gene was shown to be induced by phosphate starvation and thus negatively regulated by the pst operon. Interestingly, the putative M. smegmatis PhoA has a hydrophobic N-terminal domain which resembles a lipoprotein signal sequence. The M. smegmatis PhoA was demonstrated to be an exported protein associated with the cell surface. Furthermore, immunoprecipitation of PhoA from [(14)C]acetate-labeled M. smegmatis cell lysates demonstrated that this phosphatase is a lipoprotein.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.