ArticleJournal of bacteriology2003
Autoinducer 2 production by Streptococcus gordonii DL1 and the biofilm phenotype of a luxS mutant are influenced by nutritional conditions.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
47 citing papers in PubMed, 128 citations in OpenAlex.
- Autoinducer 2 as a universal language in microbial consortia: decoding molecular mechanisms, ecological impacts, and application.Gut microbes · 2026Review
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- The Type 9 Secretion System Is Required forFrontiers in microbiology · 2021Article
- We Are One: Multispecies Metabolism of a Biofilm Consortium and Their Treatment Strategies.Frontiers in microbiology · 2021Review
- Deciphering Streptococcal Biofilms.Microorganisms · 2020Review
- Genetics ofMicrobiology spectrum · 2019Article
- Effect of SrtA on Interspecies Adherence of Oral Bacteria.Current medical science · 2018Article
- The Impairment of Methyl Metabolism FromFrontiers in microbiology · 2018Article
- Autoinducer-2 detection among commensal oral streptococci is dependent on pH and boric acid.Journal of microbiology (Seoul, Korea) · 2016Article
- Article
- The interaction between Streptococcus spp. and Veillonella tobetsuensis in the early stages of oral biofilm formation.Journal of bacteriology · 2015Article
- Integration of Metabolic and Quorum Sensing Signals Governing the Decision to Cooperate in a Bacterial Social Trait.PLoS computational biology · 2015Article
- AI-2 of Aggregatibacter actinomycetemcomitans inhibits Candida albicans biofilm formation.Frontiers in cellular and infection microbiology · 2014Article
- Quorum-sensing systems LuxS/autoinducer 2 and Com regulate Streptococcus pneumoniae biofilms in a bioreactor with living cultures of human respiratory cells.Infection and immunity · 2013Article
- Heterologous expression of sahH reveals that biofilm formation is autoinducer-2-independent in Streptococcus sanguinis but is associated with an intact activated methionine cycle.The Journal of biological chemistry · 2012Article
- Autoinducer-2 influences interactions amongst pioneer colonizing streptococci in oral biofilms.Microbiology (Reading, England) · 2012Article
- LuxS mediates iron-dependent biofilm formation, competence, and fratricide in Streptococcus pneumoniae.Infection and immunity · 2011Article
- The LuxS-dependent quorum-sensing system regulates early biofilm formation by Streptococcus pneumoniae strain D39.Infection and immunity · 2011Article
- Investigation of supragingival plaque microbiota in different caries status of Chinese preschool children by denaturing gradient gel electrophoresis.Microbial ecology · 2011Article
- Production of cell-cell signalling molecules by bacteria isolated from human chronic wounds.Journal of applied microbiology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
The luxS gene, present in many bacterial genera, encodes the autoinducer 2 (AI-2) synthase. AI-2 has been implicated in bacterial signaling, and this study investigated its role in biofilm formation by Streptococcus gordonii, an organism that colonizes human tooth enamel within the first few hours after professional cleaning. Northern blotting and primer extension analyses revealed that S. gordonii luxS is monocistronic. AI-2 production was dependent on nutritional conditions, and maximum AI-2 induction was detected when S. gordonii was grown in the presence of serum and carbonate. In planktonic cultures, AI-2 production rose sharply during the transition from exponential to stationary phase, and the AI-2 concentration peaked approximately 4 h into stationary phase. An S. gordonii luxS mutant that did not produce AI-2 was constructed by homologous recombination. Complementation of the mutant by insertion of an intact luxS gene into the chromosome in tandem with the disrupted gene restored AI-2 production to a level similar to that of the wild-type strain. In planktonic culture, no growth differences were observed between the mutant and wild-type strains when five different media were used. However, when grown for 4 h as biofilms in 25% human saliva under flow, the luxS mutant formed tall microcolonies that differed from those formed by the wild-type and complemented mutant strains. Biofilms of the luxS mutant exhibited finger-like projections of cells that extended into the flow cell lumen. Thus, the inability to produce AI-2 is associated with altered microcolony architecture within S. gordonii biofilms formed in saliva during a time frame consistent with initial colonization of freshly cleaned enamel surfaces.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.